Twenty marine actinomycetes were isolated from sediments and rhizosphere of some halophyte plants from coastal regions of North Delta, Egypt. Four isolates which showed a wide range of antimicrobial activities (inhibition for both Gram-positive and Gram-negative bacteria, and fungi) were selected and identified on the basis of their cellular morphology, physiological and chemotaxonomic characterization. The isolates were identified and named as Streptomyces albus strain DEG18, Streptomyces canaries strain REB9, Streptomyces sp strain REB5 and Streptomyces sp strain G12. Extraction of metabolites filtrate and biomass were carried out by ethyl acetate and acetone, respectively. In secondary screening, all four Streptomyces strains showed antibacterial activity against Enterobacter cloaca and antifungal activity toward Fusarium oxysporum, three strains out of them showed antibacterial activity against Bacillus cereus, whereas two showed activity against Bacillus subtilis and Staphylococcus aureus, whereas some strains showed activity toward Klebsiella pneumoniae and Alternaria alternata.