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279741

Molecular Mechanisms of Fosfomycin Resistance in MDR Escherichia coli Isolates from Urinary Tract Infections

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Escherichia coli is one of the most common pathogens in nosocomial and community-acquired infections in humans. Fosfomycin, a broad-spectrum antibiotic, prevents the production of peptidoglycan, which is necessary for developing bacterial cell walls. Urinary Tract Infections (UTIs) remain the most prevalent infectious diseases in hospital and community settings. In Egypt, E. coli is the most frequent microbe involved in UTIs. Unfortunately, Egypt has comparatively higher levels of fosfomycin resistance among Uropathogenic E. coli (UPEC) strains. Objective: we investigated the molecular mechanisms of fosfomycin resistance in seven previously reported MDR clinical isolates of E. coli. Methodology: we initially explored the tested isolates for the three plasmid-conjugated genes: fosA3, fosC2, and fosA presence. Isolates that did not show any of the three genes were then examined for mutations in the chromosomal genes: murA, glpT, and uhpT. Results: confirm that five out of the seven tested isolates were positive for the fosA3gene. No detection for fosC2 or fosA genes in any of these isolates. Mutations in murA and glpT were confirmed in the other two isolates (isolates UPEC 9 and UPEC 27). MurA and GlpT proteins showed new amino acid substitutions. Conclusion: we herein report that fosfomycin resistance in the tested UPEC isolates were due to unique amino acid changes in MurA or the loss of function of the transporters.

DOI

10.21608/ejmm.2023.279741

Keywords

Mutation, FosA 3, MurA, UhpT

Authors

First Name

Rehab

Last Name

Bahy

MiddleName

-

Affiliation

Microbiology and Immunology Faculty of Pharmacy Fayoum university- Fayoum-Egypt

Email

rhb11@fayoum.edu.eg

City

Giza

Orcid

-

First Name

Wael

Last Name

Abu El-Wafa

MiddleName

Mohamed

Affiliation

Department of Microbiology, General Division of Basic Medical Sciences, Egyptian Drug Authority (EDA), Formerly National Organization for Drug Control and Research (NODCAR), 6 Abou Hazem St., Pyramids Ave., Giza12611, Egypt.

Email

waelnooreldeen@yahoo.com

City

Giza

Orcid

-

First Name

Ahmed

Last Name

Abouwarda

MiddleName

Megahed

Affiliation

Department of Microbiology, General Division of Basic Medical Sciences, Egyptian Drug Authority (EDA), Formerly National Organization for Drug Control and Research (NODCAR), 6 Abou Hazem St., Pyramids Ave., Giza12611, Egypt.

Email

ahmedabouwarda@gmail.com

City

Giza

Orcid

0000-0001-8745-8628

Volume

32

Article Issue

2

Related Issue

38873

Issue Date

2023-04-01

Receive Date

2023-01-11

Publish Date

2023-04-01

Page Start

25

Page End

29

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 2_Pages 25-29.pdf

PDF . 258.1kB

Link

https://ejmm.journals.ekb.eg/article_279741.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=279741

Order

279,741

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Molecular Mechanisms of Fosfomycin Resistance in MDR Escherichia coli Isolates from Urinary Tract Infections

Details

Type

Article

Created At

23 Jan 2023