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279737

Line Probe Assay for Rapid Diagnosis of Multidrug Resistant Pulmonary Tuberculosis

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Last updated: 23 Jan 2023

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Abstract

Multidrug-resistant tuberculosis (MDR-TB) is a serious public health problem with ultimate concern in the developing countries, including Egypt. In the global TB report issued in 2021 by World Health Organization (WHO), infection caused by the tubercle bacilli Mycobacterium tuberculosis (MTB) represents the 13th chief cause of mortality worldwide. Effective MDR-TB control counts on the accuracy and rapidity of its diagnosis in order to start the proper treatment regimen and as a result limit the transmission of the resistant isolate. Line probe assay (LPA) and GeneXpert MTB/RIF are two WHO approved probe based molecular assays for rapid detection resistant MTB isolates via screening its nucleic acid for any mutations. For the purpose of finding mutations linked to rifampicin resistance using DNA probes, both techniques focus on the same 81 bp Rifampicin Resistance Determining Region. Line probe assay can detect both rifampicin and isoniazid resistance, thus can detect isoniazid mono resistant isolates, which requires special management protocol, in addition to detecting MDR-TB isolates as well with high sensitivity and specificity.

DOI

10.21608/ejmm.2023.279737

Keywords

Multidrug-resistant tuberculosis (MDR-TB), Line probe assay (LPA), GeneXpert MTB/RIF (Xpert)

Authors

First Name

Nancy

Last Name

Bassuony

MiddleName

Abdulmonem

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Email

nancybassuony@mans.edu.eg

City

Mansoura

Orcid

https://orcid.org/00

First Name

Wafaa

Last Name

Zaghloul

MiddleName

Saad

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University.

Email

wafaa.saad628@gmail.com

City

-

Orcid

-

First Name

Mohammad

Last Name

Abou Elela

MiddleName

Ahmad

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University

Email

abouelela@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Elbadrawy

MiddleName

Khairy

Affiliation

Professor of Chest Medicine, Chest Medicine Department, Faculty of Medicine, Mansoura University

Email

profkhairy@yahoo.com

City

-

Orcid

-

First Name

Dalia

Last Name

Moemen

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology

Email

dr_daliamoemen@yahoo.com

City

-

Orcid

0000-0002-4103-3840

Volume

32

Article Issue

2

Related Issue

38873

Issue Date

2023-04-01

Receive Date

2023-01-11

Publish Date

2023-04-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_279737.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=279737

Order

279,737

Type

Review articles

Type Code

2,039

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

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Article

Created At

23 Jan 2023