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277792

Expression of Autophagy Related Genes mTOR, ATG10 and P62 in The Peripheral Blood Mononuclear Cells of Systemic Lupus Erythematosus Egyptian Patients

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Autoimmune diseases are knowen to be associated with abnormalities in autophagy. Objectives:  In our study we evaluated the expression of autophagy related genes mTOR, P62 and ATG10 in the peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) and assess their relationship with disease activity and immunologic features. Methodology: The expression of mTOR, P62and ATG10 was detected by RT-PCR in 70 SLE subjects and 30 age- and sex-matched healthy controls. Data regarding demographics and clinical parameters were collected. Disease activity of SLE was evaluated according to the SLE Disease Activity Index (SLEDAI) score. Mann-Whitney test was used to analyze the expression of mTOR, P62 and ATG10 in the two groups. Spearman's correlation was performed to analyze their relationship with disease activity and immunologic features. Results: The median and IQ (interquartile range) levels of P62 and ATG10 mRNA were significantly higher in SLE patients than the controls 1.11, (0.34-8.59) vs 0.25, (0.01-1.0) for P62 with P<0.002; 2.24 (0.55-19.31) vs 0.77 (0.02-1.0) for ATG10 with P<0.000). However, the mRNA relative expression level of mTOR in PBMCs was significantly very high in controls than cases of SLE 1.0, (1.0-2.14) vs0.31 ,(0.14-0.89) with P< 0.000). Conclusion: Correlation analysis showed that mTOR mRNA levels only correlated negatively with SLEDAI; also our research measure serum level of IL17 in the study population of cases and controls.

DOI

10.21608/ejmm.2023.277792

Keywords

Systemic lupus exrythematosus, autophagy, mTOR, p62, ATG10

Authors

First Name

Ekram

Last Name

Abd Rhaman

MiddleName

Mahmoud

Affiliation

medical micobiology and immunology faculty of medicine sohag university

Email

dr.ekram.ar@gmail.com

City

sohag

Orcid

-

First Name

Essam

Last Name

Abu Alfadl

MiddleName

Mohammed

Affiliation

Rheumatology and Rehabilitation Faculty of Medicine, Sohag University

Email

dndn88@yahoo.com

City

sohag

Orcid

-

First Name

Dina

Last Name

Mohammed

MiddleName

Hamada

Affiliation

Medical microbiology and immunology Department. Faculty of Medicine Sohag University

Email

dinamahrous41@gmail.com

City

-

Orcid

-

First Name

Abeer

Last Name

Sheneef

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Sohag University

Email

dr_ab_sh@yahoo.com

City

sohag

Orcid

-

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

133

Page End

140

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 133-140.pdf

PDF . 393.7kB

Link

https://ejmm.journals.ekb.eg/article_277792.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277792

Order

277,792

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Expression of Autophagy Related Genes mTOR, ATG10 and P62 in The Peripheral Blood Mononuclear Cells of Systemic Lupus Erythematosus Egyptian Patients

Details

Type

Article

Created At

23 Jan 2023