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277787

Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient

Article

Last updated: 23 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Multidrug resistant infection is the leading cause of ICU morbidity and mortality. Gram-negative bacilli are the most prevalent pathogens with Acinetobacter being the most resistant. Objectives: Phenotypic and genotypic characterization of multidrug- and extensively drug-resistant Acinetobacter and Enterobacteriaceae species causing infections in critically ill patients with detection of targeted β-lactamase genes and its impact on patients' morbidity and mortality. Methodology: Antibiotic susceptibility testing was done. Genes for class A and class B β-lactamases were detected by multiplex polymerase chain reaction (PCR) and 5 carbapenemases genes were detected by real time PCR using GeneXpert Carba-R system. Results: 16.6 % of A. baumannii were MDR, 50% were XDR, 50% of K. pneumoniae were XDR and 100% of P. stuartii were XDR. Detected genes included blaSHV, blaCTX-M, blaGIM-1, blaSIM-1, blaOXA-48 and blaNDM-1. Conclusion: MDR/XDR infections had a significant impact on patients' mortality. Molecular epidemiology is crucial to guide infection control committees about preventive measures that can contain MDR/XDR infections and prevent their detrimental effect on patients' outcome.

DOI

10.21608/ejmm.2023.277787

Keywords

Antimicrobial, resistance, Gram-Negative, Carbapenemase, carbapenems

Authors

First Name

Reham

Last Name

Khalifa

MiddleName

Ali

Affiliation

Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University

Email

drreham_khalifa@med.asu.edu.eg

City

-

Orcid

0000-0003-3012-1164

First Name

Hani

Last Name

Aljahdali

MiddleName

-

Affiliation

Medical Department, King Abdulaziz Hospital, Jeddah, Saudia Arabia

Email

haljahdali@moh.gov.sa

City

Jeddah

Orcid

-

First Name

Huda

Last Name

Alghamdi

MiddleName

-

Affiliation

Laboratory Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia

Email

hudaalghamdi04@hotmail.com

City

Jeddah

Orcid

-

First Name

Bothayna

Last Name

Ismail

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Medicine, Al Azhar University, Egypt.

Email

salehb08@gmail.com

City

Cairo

Orcid

-

First Name

Husam

Last Name

Joharjy

MiddleName

-

Affiliation

Infection Control Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia

Email

hjoharjy@moh.gov.sa

City

Jeddah

Orcid

-

First Name

Khalid

Last Name

Eibani

MiddleName

-

Affiliation

Surgery Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia

Email

keibani@moh.gov.sa

City

-

Orcid

-

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

113

Page End

123

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 113-123.pdf

PDF . 698.3kB

Link

https://ejmm.journals.ekb.eg/article_277787.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277787

Order

277,787

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023