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277784

SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background: SARS-CoV-2 identification via Real-time polymerase chain reaction (RT-PCR) testing considered the current standard for the diagnosis of infection, but its utilization for large-scale screening is limited. Serological immunoglobulin M (IgM)/IgG testing has been proposed as a useful tool for detecting SARS-CoV-2 exposure, Objectives; to assess the diagnostic value of SARS-COV-2 IgM and IgG in early diagnosis of patient with COVID-19 versus viral RNA detection by PCR and to Investigate the sensitivity of nasopharyngeal swabs versus oropharyngeal samples for early detection of SARS-COV-2. Methodology:  This study was carried on 200 COVID-19 PCR positive cases divided into two groups as (Group I). In addition to 200 apparently healthy individuals (Negative PCR) as a control group (Group II) obtained From Ministry of Health Hospitals after getting approval. All patients were subjected to: clinical examination, lab investigation including; SARS-COV-2 IgM and IgG testing, SARS-COV-2 PCR test from nasopharyngeal and Oropharyngeal swabs samples, Results; Regarding IgM after 10 days, It was positive in 77 (38.5 %) of patients with Positive PCR while no one was positive in patients with negative PCR with sensitivity of 38.5 % , Specificity 100.0 %, and accuracy 69.25 %, Nasopharyngeal Swabs (NPS) had significantly higher SARS-CoV-2 detection rate, sensitivity, and viral load than Oropharyngeal Swabs. NPS could reduce droplets production during swabs. Conclusion: NPS should be recommended for diagnosing COVID-19 and monitoring SARS-CoV-2 load. Also, our study analyzed the clinical performance of the rapid serological test and confirmed the test's limited applicability for the diagnosis of SARS-CoV-2 infection.

DOI

10.21608/ejmm.2023.277784

Keywords

COVID-19, Oropharyngeal and Nasopharyngeal swabs, PCR, Serological Tests

Authors

First Name

fatma

Last Name

khalil

MiddleName

o

Affiliation

Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom, Egypt

Email

fatma.khalil@liver.menofia.edu.eg

City

shebin el-kom

Orcid

0000-0002-3157-5232

First Name

Azza

Last Name

Abd ElAziz

MiddleName

-

Affiliation

Departement of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University Menoufia, Egypt

Email

azzazoze@gmail.com

City

Menoufia

Orcid

-

First Name

mohamed

Last Name

Abedelrahman

MiddleName

-

Affiliation

Medical Military Academy, Alexandria, Egypt

Email

mohammad_rhman1963@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Abedelrahman

MiddleName

-

Affiliation

Medical Military Academy, Alexandria, Egypt

Email

ahmedmousanavy@gmail.com

City

-

Orcid

-

First Name

Tawfik

Last Name

Abed El motaleb

MiddleName

Mohamed

Affiliation

Professor of Medical Microbiology and Immunology, Clinical Microbiology and Immunology department, National Liver Institute, Menoufia University, Shebin El-kom 32511, Egypt,

Email

tawfeek.mohamed@liver.menofia.edu.eg

City

Shebin elkom

Orcid

-

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

87

Page End

95

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 87-95.pdf

PDF . 350.4kB

Link

https://ejmm.journals.ekb.eg/article_277784.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277784

Order

277,784

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

SARS-COV-2 antibody testing Versus Nasopharyngeal and Oropharyngeal swab Sample Polymerase Chain Reaction for Diagnosis of Coronavirus Disease 2019 (COVID-19) in Egyptian Patient. A Comparative study

Details

Type

Article

Created At

23 Jan 2023