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277782

Rapid Disc Diffusion Antibiotic Susceptibility Testing for Gram Negative Non- fermenters: Pseudomonas and Acinetobacter

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Non fermentative Gram-negative bacilli are opportunistic pathogens associated with serious hospital infections. Antibiotic susceptibility pattern of these pathogens has become unpredictable and disc diffusion remains the preferred standardized, most used and cost-effective method according to the CLSI and EUCAST. However, the method is standardized for overnight incubation (16-18 hrs.), which delays result an extra day. Objective: The aim of our study was to examine the possibility and accuracy of manually interpreting disc diffusion zone diameter results for clinical isolates of Acinetobacter species and Pseudomonas species, after 6 hrs. and 8 hrs. incubation, in comparison to the standard overnight incubation. Results: This study showed that there was a good level of agreement for early zone measurement of Acinetobacter AST after 6 hrs., which improved with extending incubation to 8 hrs. As for Pseudomonas species, zone measurement at 8 hrs. resulted in minor errors of 10.4% and very major errors of 1.2%, which just exceeds the guideline allowed limits. Conclusion: Rapid antibiotic susceptibility testing and interpretation was manually possible. Both Acinetobacter and Pseudomonas were measurable at 8 hrs., but Acinetobacter measurements were more accurate with minimal errors, which makes it a promising cost-effective method for rapid delivery of AST results for Acinetobacter. These findings are worthy of further studies, to determine the best incubation time that would allow rapid results delivery with minimal errors for different bacterial species.

DOI

10.21608/ejmm.2023.277782

Keywords

Rapid Disc Diffusion, Antibiotic susceptibility, Acinetobacter, Pseudomonas

Authors

First Name

Hadir

Last Name

Okasha

MiddleName

A.S.

Affiliation

Assistant Professor Medical Microbiology & Immunology Department, Faculty of Medicine, Alexandria University, Egypt

Email

hadir.okasha@alexmed.edu.eg

City

Alexandria

Orcid

-

First Name

Sara

Last Name

Asser

MiddleName

Lotfy

Affiliation

Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University

Email

sara.asar@alexmed.edu.eg

City

Alexandria

Orcid

0000-0002-9937-9473

First Name

Dina

Last Name

Kholeif

MiddleName

Aly

Affiliation

Lecturer of Medical Microbiology and Immunology Department, Alexandria University, Egypt

Email

dina.kholeif@alexmed.edu.eg

City

Alexandria

Orcid

-

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

77

Page End

86

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 77-86.pdf

PDF . 471.8kB

Link

https://ejmm.journals.ekb.eg/article_277782.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277782

Order

277,782

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Rapid Disc Diffusion Antibiotic Susceptibility Testing for Gram Negative Non- fermenters: Pseudomonas and Acinetobacter

Details

Type

Article

Created At

23 Jan 2023