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277776

Detection of Mutations Associated with Resistance to Second-line Drugs in Isolates of Mycobacterium tuberculosis

Article

Last updated: 23 Jan 2023

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Subjects

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Tags

Diagnostics

Abstract

Background: Mycobacterium tuberculosis (M. tuberculosis) develops resistance toward second-line anti-tuberculous drugs, mostly through mutations of the chromosome. The mechanism of resistance is complicated and accompanies several genes as rrs and eis promoter mutations. Objective: To assess the validity of the Real-time PCR (Rt-PCR) in recognizing the mutations of the resistance to second-line injectable agents (capreomycin, amikacin and kanamycin) in multidrug resistant tuberculosis (MDR-TB) clinical isolates in comparison with the gold standard proportion method (PM) using Lowenstein–Jensen (LJ) media. Methodology: This study was conducted on 48 MDR-TB clinical isolates (from sputum and bronchioalveolar lavage samples) obtained from the Egyptian National Central Laboratory of the Ministry of Health, Egypt. The isolates obtained were resistant to a minimum one of the second-line anti-tuberculous drugs (ofloxacin, capreomycin, amikacin, and kanamycin) identified by PM using LJ media. Isolates were tested by Rt-PCR to track mutations in rrs and the eis promoter. Results: Thirty-eight (38) isolates were positive for rrs, and 32 isolates for the promoter of eis using Rt-PCR. Comparing the results to the gold standard PM, an agreement of 100%, and 69% were found to rrs, and the eis promoter, respectively. Conclusion: Using the Real-time PCR for recognizing mutations related to second-line anti-tuberculous drugs highly agrees with the PM. This could help in MDR-TB early detection and screening for extensively drug-resistant TB (XDR-TB) strains.

DOI

10.21608/ejmm.2023.277776

Keywords

M. tuberculosis, MDR-TB, RT-PCR, rrs, promoter of eis

Authors

First Name

Fatma EL Zahraa

Last Name

Fathi

MiddleName

Yousef

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Email

fruitty_21@hotmail.com

City

-

Orcid

-

First Name

Amany

Last Name

Kamal

MiddleName

Mostafa

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Email

dr_amany_mostafa@yahoo.com

City

-

Orcid

-

First Name

Makram

Last Name

Attallah

MiddleName

Fahmy

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Email

f_makram@med.asu.edu.eg

City

-

Orcid

-

First Name

Noha

Last Name

Salah El Deen

MiddleName

Nagi

Affiliation

Medical Microbiology and Immunology, Faculty of medicine, Ain shams University, Cairo, Egypt

Email

dr.noha_nagi@hotmail.com

City

-

Orcid

-

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

47

Page End

53

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 47-53.pdf

PDF . 507.9kB

Link

https://ejmm.journals.ekb.eg/article_277776.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277776

Order

277,776

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023