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277771

Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Carbapenemase-producing Enterobacterales are increasingly spreading in healthcare facilities. Identifying the type of carbapenemase can help epidemiologic surveillance and proper selection of antimicrobials. Objective: This study assessed the sensitivity and specificity of carbapenem inactivation method (mCIM with eCIM) for identification of carbapenemase-production. Methodology: The study involved 150 isolates of Enterobacterales. Carbapenem-resistant isolates by Kirby Bauer method were further tested for carbapenemase production phenotypically by mCIM with eCIM, and genotypically by multiplex PCR using specific primers for blaKPC, blaOXA-48, blaNDM-1, blaVIM, and blaIMP. Results: Resistance to carbapenem was observed in 53/150 isolates. Phenotypically, 28/53 isolates produced metallo-β-lactamase, 16/53 produced serine carbapenemase, 5/53 isolates gave inconclusive results, and 4/53 were negative by mCIM with eCIM test. Genotypically, 30 isolates carried blaNDM-1, and 41 isolates carried blaOXA-48. Both genes co-existed in 18 Metallo-β-lactamase producers. The 9 isolates with negative or inconclusive results carried carbapenemase-encoding genes. For mCIM with eCIM test the sensitivity and specificity of detecting Metallo-β-lactamase production were higher (87% and 91%) than for serine carbapenemase detection (34% and 83%), respectively. Conclusion: It was concluded that the mCIM with eCIM test does not always lead to true conclusions about the existence and the type of carbapenemase produced by Enterobacterales.

DOI

10.21608/ejmm.2023.277771

Keywords

Carbapenem-resistant Enterobacterales, mCIM with eCIM, metallo-beta-lactamase detection, serine-carbapenemase detection, blaOXA-48 gene, blaNDM-1 gene

Authors

First Name

Aliaa

Last Name

Aboulela

MiddleName

Gamaleldin

Affiliation

Medical Research Institute, Alexandria University

Email

aliaagamaleldin@alexu.edu.eg

City

Alexandria

Orcid

0000-0002-2923-1027

First Name

Mawj

Last Name

Jabbar

MiddleName

Fahad

Affiliation

High Institute of public health, Alexandria University

Email

mawj.fahad.95@gmail.com

City

Alexandria

Orcid

0000-0001-5481

First Name

Abdelfattah

Last Name

Hammouda

MiddleName

-

Affiliation

Microbiology department, High Institute of Public Health, Alexandria University, Alexandria, Egypt

Email

hiph.ahammouda@alexu.edu.eg

City

Alexandria

Orcid

0000-0001-5421-2998

First Name

Medhat

Last Name

Ashour

MiddleName

Saber

Affiliation

Microbiology department, High Institute of Public Health, Alexandria University, Alexandria, Egypt

Email

hiph.mashour@alexu.edu.eg

City

Alexandria

Orcid

0000-0002-8957-358X

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

37

Page End

46

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 37-46.pdf

PDF . 451.4kB

Link

https://ejmm.journals.ekb.eg/article_277771.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277771

Order

277,771

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales

Details

Type

Article

Created At

23 Jan 2023