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277770

IL-13 rs20541 (R130Q) Single Nucleotide Polymorphism in a Sample of Egyptian Children Suffering from Idiopathic Nephrotic Syndrome

Article

Last updated: 23 Jan 2023

OverviewSimilar Items

Subjects

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Tags

Genetics

Abstract

Background: Idiopathic nephrotic syndrome (INS) is the most frequent glomerular disease affecting children. Its pathogenesis is not completely evident, it is probably due to immunological disturbance that increases cytokines production and alters the glomerular permeability. Interleukin 13 (IL-13) has been implicated in INS pathogenesis by changing the permeability of the glomerular basement membrane and inducing proteinuria. Objective: To investigate the association between rs20541 (R130Q) single nucleotide polymorphism (SNP) in IL-13 gene, idiopathic nephrotic syndrome susceptibility and steroid treatment response in Egyptian children. Methodology: This cross sectional case-control study was performed on 50 INS children aged 2-15 years following up in the Nephrology Unit of the Pediatric department at Benha University Hospitals and 50 healthy age and sex matched children as controls. All candidates were subjected to clinical evaluation. Genotyping of the selected SNP was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: A highly significant frequency of GA genotype, p=0.017, OR= 1.933 (1.126-3.319) and AA genotype, p=0.001, OR=6.660 (2.283-19.433) as well as A allele, p<0.001, OR=2.281(1.551-3.354) were observed in the INS patients with a higher significant frequency of AA type p=0.013, OR=3.660 (1.308-10.238) and A allele, p=0.009, OR=1.947 (1.178-3.218)  in steroid resistant patients. Conclusion: The results showed that IL-13 polymorphism (rs20541) seems to play a role in the pathogenesis of INS. The A allele is a risk factor for the disease and moreover, it has a relation to steroid responsiveness.

DOI

10.21608/ejmm.2023.277770

Keywords

INS, IL, 13, R130Q SNP, rs20541, PCR, RFLP

Authors

First Name

Marwa

Last Name

EL-Melouk

MiddleName

Seif

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt.

Email

dr.marwaseif@gmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Sobeih

MiddleName

Ata

Affiliation

Pediatric Department, Faculty of Medicine, Benha University, Benha, Egypt.

Email

drahmadata2007@yahoo.com

City

-

Orcid

-

First Name

Maha

Last Name

Osman

MiddleName

Mamdouh

Affiliation

Clinical and chemical pathology, Faculty of medicine, Helwan University, Egypt.

Email

maha.osman@fmed.bu.edu.eg

City

-

Orcid

-

First Name

Hasnaa

Last Name

Abd AL-Hamid

MiddleName

Shawky

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt.

Email

ahmed_sayedb@yahoo.com

City

-

Orcid

-

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

29

Page End

35

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 29-35.pdf

PDF . 425.7kB

Link

https://ejmm.journals.ekb.eg/article_277770.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277770

Order

277,770

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023