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277767

Species Identification of Candida Isolates from Critically Ill Patients by Rapid Commercial and Genotypic Methods

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background: Rapid, reliable Candida species (spp.) identification is crucial because it enables prompt and effective antifungal treatment. Unfortunately this is difficult due to a lack of knowledge, adequate reagents, and equipments, particularly in countries with limited resources. Objectives: The aim of this study was to assess the effectiveness of rapid commercial methods in identifying Candida spp. isolated from critically ill patients. Methodology: Various clinical specimens were collected from critically ill patients admitted to the Intensive Care Units of Mansoura University Hospitals, and processed by numerous mycological techniques. Gram stained, and lactophenol cotton blue stained films, as well, germ tube test, and corn meal agar with 1% Tween 80 were used to identify the isolated Candida spp. Three commercial methods were used for spp. identification; CHROMagarTM Candida medium, API 20 C AUX and Vitek-2 Compact automated system. In addition, all Candida isolates were subjected to species-specific multiplex PCR as the reference method. Results: A total of 52 Candida strains were identified where C. albicans was the predominant spp. (53.8%) followed by C. parapsilolsis (17.3%), C. glabrata (13.5%), C. dubliniensis (5.8%), C.  tropicalis (5.8%), C. auris (1.9%) and C. krusei (1.9%). Both CHROMagarTM Candida medium and Vitek-2 Compact automated system exhibited high sensitivity and specificity rates in identification of various Candida spp. as compared to multiplex PCR. Conclusion: This study demonstrated a good performance of both CHROMagarTM Candida medium and Vitek-2 Compact automated system; however, CHROMagarTM Candida medium may be an easier and more cost-effective choice to identify Candida spp., mainly in areas with restricted resources.

DOI

10.21608/ejmm.2023.277767

Keywords

Candida albicans, CHROMagarTM Candida, Vitek-2 system, ICU, non-albicans Candida

Authors

First Name

Rasha

Last Name

Elnagar

MiddleName

Mokhtar

Affiliation

Medical microbiology and immunology, faculty of medicine, Mansoura university

Email

drrasha_m@mans.edu.eg

City

-

Orcid

0000-0002-5661-850X

First Name

Amira

Last Name

Sultan

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

amira110sultan@yahoo.com

City

Mansoura

Orcid

0000-0002-6707-9599

First Name

Mohammad

Last Name

Ahmed

MiddleName

A.

Affiliation

Department of Anesthesia and Surgical Intensive Care , Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Email

r_m8445@yahoo.com

City

Mansoura

Orcid

0000-0002-0688-421X

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

19

Page End

27

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 19-27.pdf

PDF . 493.0kB

Link

https://ejmm.journals.ekb.eg/article_277767.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277767

Order

277,767

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Species Identification of Candida Isolates from Critically Ill Patients by Rapid Commercial and Genotypic Methods

Details

Type

Article

Created At

23 Jan 2023