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277762

Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Early detection of bacterial infection in patients with cirrhosis remains a hope for improved prognosis to decrease the morbidity and mortality rate. Objective: To study the diagnostic importance of Neutrophil gelatinase-associated lipocalin-2 and macrophage antigen -1 for early detection of infection in cirrhotic patients and their relationship with carbapenem resistant bacteria. Methodology: 150 patients were divided into group I: cirrhotic patients complicated with infection subdivided into three subgroups; A: fifty-five patients with spontaneous bacterial peritonitis (SBP), Subgroup B: fifteen patients with culture-negative neutrocytic ascites (CNNA) and subgroup C: thirty patients with urinary tract infection. Group II: fifty cirrhotic patients without infections. NGAL-2 estimation was done by ELISA, neutrophil MAC-1 by flowcytometry and Carbapenem resistance genes; OXA-48 and KPC-3 were detected by RFLP-PCR analysis Results: Significant increase of ascitic fluid neutrophils and WBCs count in SBP subgroup. Mean fluorescence intensity of Mac-1 and NGAL-2 concentrations were significantly higher in cirrhotic infected than uninfected patients. The OXA-48 and KPC-3 Carbapenem resistant gram-negative bacteria were prevalent among cirrhotic infected patients. NGAL-2 can detect early infection in cirrhotic patients followed by Mac-1. Conclusion: NGAL-2 and Mac-1 could be used for early detection of bacterial infection in cirrhotic patients with high specificity and sensitivity.

DOI

10.21608/ejmm.2023.277762

Keywords

Mac-1, NGAI-2, SBP, PCR, flow cytometer, Cirrhosis

Authors

First Name

fatma

Last Name

khalil

MiddleName

o

Affiliation

Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom, Egypt

Email

fatma.khalil@liver.menofia.edu.eg

City

shebin el-kom

Orcid

0000-0002-3157-5232

First Name

Sally Saad Mandour

Last Name

Mandour

MiddleName

Saad

Affiliation

Clinical Pathology Department, National Liver Institute Menoufia University

Email

sallysaad@liver.menofia.edu.eg

City

-

Orcid

-

First Name

Isis

Last Name

Bedira

MiddleName

Samy

Affiliation

Clinical Pathology Department, National Liver Institute Menoufia University

Email

isis.samy850@liver.menofia.edu.eg

City

-

Orcid

-

First Name

Sally

Last Name

Elkhadry

MiddleName

-

Affiliation

Epidemiology and Preventive Medicine, National Liver Institute Menoufia University

Email

drsallywaheed@liver.menofia.edu.eg

City

-

Orcid

0000-0001-9305-3045

First Name

Amr

Last Name

Ibrahim

MiddleName

Ragab

Affiliation

Hepatology and Gastroenterology Department, National Liver Institute Menoufia University

Email

amr.ragab124@yahoo.com

City

-

Orcid

-

First Name

Neamat

Last Name

Abdelmageed

MiddleName

-

Affiliation

5Department of Hepatogastroentrology & Infectious Diseases, Faculty of Medicine for girls, Al-Azhar University

Email

dr.neamatabdelmageed@yahoo.com

City

-

Orcid

-

First Name

Eman

Last Name

El -shemy

MiddleName

-

Affiliation

5Department of Hepatogastroentrology & Infectious Diseases, Faculty of Medicine for girls, Al-Azhar University

Email

dr.emanelshemy@gmail.com

City

-

Orcid

-

First Name

Hala

Last Name

El-refai

MiddleName

Ahmed

Affiliation

Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom

Email

halabazeed@gmail.com

City

shebin el-kom

Orcid

-

Volume

32

Article Issue

1

Related Issue

38243

Issue Date

2023-01-01

Receive Date

2023-01-01

Publish Date

2023-01-01

Page Start

1

Page End

11

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 1_Pages 1-11.pdf

PDF . 562.6kB

Link

https://ejmm.journals.ekb.eg/article_277762.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=277762

Order

277,762

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae

Details

Type

Article

Created At

23 Jan 2023