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264208

Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Staphylococcus aureus (S. aureus) with the incomplete hemolytic phenotype (SIHP) is known for its dark hemolytic ring, which differs from the transparent S. aureus with complete hemolytic phenotype (SCHP). SIHP is recently linked to severe infections and antimicrobial resistance. Panton-Valentine leucocidin (PVL) and hemolysin are documented virulence factors for S. aureus infection. Objectives: We conducted this study to recognize methicillin-resistant S. aureus (MRSA) strains with SIHP phenotype and evaluate its association with the PVL and hemolysin in patients with bloodstream infections (BSIs). Methodology: Ninety-Three S. aureus isolates were recognized during the study period, and they were evaluated for resistance to thirteen antibiotics. Blood agar plates were used to culture these isolates, and they underwent ten passages for identification of their hemolytic phenotype. PVL and hemolysin genes were tested via polymerase chain reaction (PCR). Results: SIHP constituted 34.4% of the collected isolates. PVL positivity was significantly increased in SIHP (34.4% vs. 16.4% in SCHP). Also, SIHP was strongly associated with MRSA (62.5% vs. 39.3% of SCHP). In SIHP isolates, PVL positivity was associated with MRSA (55% vs. 0 % in MSSA) and increased resistance to Augmentin, cefoxitin, and gentamycin. Conclusion: The prevalence of SIHP is increasing among S. aureus. The prevalence of the PVL gene is higher in SIHP, and it is associated with the presence of MRSA.

DOI

10.21608/ejmm.2022.264208

Keywords

Staphylococcus aureus, Incomplete hemolytic phenotype, MRSA, Blood stream infections, pvl gene

Authors

First Name

Noha

Last Name

Mostafa

MiddleName

-

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

nohamostafa_81@yahoo.com

City

Mansoura

Orcid

https://orcid.org/00

First Name

Mohammed

Last Name

Salah

MiddleName

-

Affiliation

Cardiovascular Medicine Department, Faculty of Medicine, Mansoura, Mansoura University, Egypt

Email

dr_mohammed_salah@yahoo.com

City

-

Orcid

-

First Name

Amira

Last Name

Elashrey

MiddleName

-

Affiliation

Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

dr_amirah@mans.edu.eg

City

Mansoura

Orcid

-

Volume

31

Article Issue

4

Related Issue

36977

Issue Date

2022-10-01

Receive Date

2022-10-05

Publish Date

2022-10-01

Page Start

141

Page End

149

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 4_Pages 141-149.pdf

PDF . 460.8kB

Link

https://ejmm.journals.ekb.eg/article_264208.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=264208

Order

264,208

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections

Details

Type

Article

Created At

23 Jan 2023