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Linezolid Susceptibility and Virulence Factors in Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium among Hospitalized Burn Patients

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Enterococci are the 3rd cause of HAIs. E. faecalis and E. faecium are the commonest enterococcal species, showed resistance to vancomycin due to resistance genes (vanA, vanB and vanC). Linezolid is considered a good substitute. The virulence factors like asa1, gelE, cylA, esp, and hyl may interfere with antibiotic susceptibility. Objectives: Determine linezolid resistance in VR E. faecalis and E. faecium in relation to virulence factors. Methodology: Enterococcus spp. identified by colony morphology, Gram stain, biochemical reactions and by the VITEK 2 system. Antibiotic susceptibility was done through VITEK 2 system, AST-GP72 card. Vancomycin and linezolid MIC were done according to CLSI. Multiplex PCR for ddlE. faecalis, ddlE. faecium. vanA and vanB detection. Other for asa1, gelE, cylA, esp, and hyl virulence genes determination then conventional PCR for cfr and optrA genes were done. Results: A total of 65 enterococci CIs. (45 E. faecalis & 20 E. faecium) were isolated from different samples. E. faecalis and E. faecium were resistant to vancomycin by 11,1% and 35% and to linezolid by 4.4% and 10% respectively. The vanA, vanB, cfr and optrA genes were present in 100% of VR E. faecalis like E. faecium except that, the cfr was not detected. The gelE was frequently detected in E. faecalis followed by asa1, esp, hyl and finally cylA. And for E. faecium, the most frequent one was asa1followed by gelE. esp, and finally cylA and hyl. Conclusions: LZD resistant enterococci were increasingly detected, with no significant relation between linezolid resistance and vancomycin resistance. And with different impact of virulence genes.

DOI

10.21608/ejmm.2022.262691

Keywords

Enterococcus, VRE, LZD, and Virulence

Authors

First Name

Shymaa

Last Name

Elaskary

MiddleName

Abd Elsattar

Affiliation

medical microbiology and immunology faculty of medicine Menoufia University

Email

dr.shaimaaelaskary@yahoo.com

City

-

Orcid

0000-0003-0588-9761

First Name

Eman

Last Name

Zaher

MiddleName

Mohammed

Affiliation

Clinical pathology , faculty of medicine Menoufia University

Email

zahereman2011@yahoo.com

City

Shibin El kom

Orcid

0000-0001-6186-2710

Volume

31

Article Issue

4

Related Issue

36977

Issue Date

2022-10-01

Receive Date

2022-10-01

Publish Date

2022-10-01

Page Start

71

Page End

82

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 4_Pages 71-82.pdf

PDF . 583.6kB

Link

https://ejmm.journals.ekb.eg/article_262691.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=262691

Order

262,691

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Linezolid Susceptibility and Virulence Factors in Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium among Hospitalized Burn Patients

Details

Type

Article

Created At

23 Jan 2023