Beta
ManageSearchChatDiscover
262687

Tedizolid versus linezolid activity against Staphylococcus aureus clinical isolates

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: In recent years, emergence of linezolid-resistant strains has gained a considerable clinical concern. Tedizolid, a relatively new antibiotic, showed strong effectiveness against S. aureus; however, few data are available from Egypt in this regard. Objectives: This study aimed to assess the in vitro susceptibility of S. aureus clinical isolates from Mansoura University Hospitals (MUHs), Egypt to linezolid and tedizolid, and to unveil the underlying molecular mechanisms for resistance. Methodology: In vitro susceptibility of 113 S. aureus isolates from various clinical samples was determined by disc diffusion method. The broth microdilution method was used to determine the MICs of linezolid and tedizolid. Detection of mecA and cfr genes among MRSA and linezolid-resistant isolates, respectively was performed using polymerase chain reaction (PCR). Results: In this study, 16.8% of S. aureus isolates were methicillin-resistant. On the other hand, resistance to vancomycin and linezolid was identified in 15%, and 9.7 % of the isolates, respectively. All the isolates were susceptible to tedizolid. In comparison between methicillin-sensitive S. aureus (MSSA) and MRSA as regards tedizolid and linezolid susceptibility, tedizolid retained low MIC50, and MIC90 (0.25, and 0.5 µg/L) for all S. aureus isolates. On the other hand, the MICs of linezolid were 0.5–2 µg/L for MSSA, and 0.5–>4 µg/L for MRSA isolates. All MRSA isolates (n= 19) were found to harbor the mecA gene. The cfr gene was identified in 57.9% of linezolid-resistant MRSA isolates. Conclusion: Tedizolid is highly effective against S. aureus clinical isolates, including linezolid-resistant strains.

DOI

10.21608/ejmm.2022.262687

Keywords

Linezolid resistance, Staphylococcus aureus, cfr gene, tedizolid, Minimum inhibitory concentration (MIC)

Authors

First Name

Rasha

Last Name

Elnagar

MiddleName

Mokhtar

Affiliation

Medical microbiology and immunology, faculty of medicine, Mansoura university

Email

drrasha_m@mans.edu.eg

City

-

Orcid

0000-0002-5661-850X

First Name

Rasha

Last Name

Mahmoud

MiddleName

-

Affiliation

Internal medicine department,nephrology and dialysis unit, Mansoura university

Email

drrashamahmoud73@gmail.com

City

-

Orcid

-

First Name

Rania

Last Name

El-Kady

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University

Email

raniael_kady@yahoo.com

City

-

Orcid

-

Volume

31

Article Issue

4

Related Issue

36977

Issue Date

2022-10-01

Receive Date

2022-10-01

Publish Date

2022-10-01

Page Start

53

Page End

60

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 4_Pages 53-60.pdf

PDF . 628.7kB

Link

https://ejmm.journals.ekb.eg/article_262687.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=262687

Order

262,687

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Tedizolid versus linezolid activity against Staphylococcus aureus clinical isolates

Details

Type

Article

Created At

23 Jan 2023