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262685

Ascitic Fluid Micro RNA 155 as a Biomarker for Spontaneous Bacterial Peritonitis

Article

Last updated: 23 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Spontaneous bacterial peritonitis is a life-threatening complication in cirrhotic ascitic patients. Objectives: We aimed to evaluate ascitic fluid microRNA155 level as diagnostic markers of (SBP) in association with Leukocyte esterase test, MPV, PDW, ascitic fluid CRP and culure. Methodology: A cross sectional study included 64 patients with ascites admitted to the Hepatology, Gastroenterology and Infectious diseases Department, Benha University Hospital, Egypt, divided into two groups: SBP group (32 pts.) and non SBP group (32 pts.).50 ml. of ascitic fluid was obtained for assessment of CRP, Leukocyte esterase test, bacterial culture and miR-155 and 12 ml blood for the routine laboratory test. Results: The AUC of miRNA 155 level was acceptable for discrimination between both groups (AUC=0.959), with cut off values 1.14pg/ml. Sensitivity 93.8%, specificity 93,7%,  the cut-off value of ascitic fluid CRP was ≥12 ng/ml could predict SBP with sensitivity 81.3 % and specificity 81.2%  , the Leucocyte esterase test cut-off value  grade 1 and above could predict SBP with sensitivity 96.9 % , specificity of 84. %, accuracy of 90.6% ,with (AUC ) of  0.906,  MPV and PDW could  predict SBP with  cut of ≥10.5% and 14.3fl  , sensitivity of 84.4% and 59.4%% , and specificity of 84.4% and 62.5%% ,accuracy of 84.4 % and 60.9  %, the  combined usage of rapid bed side tests including leucocyte esterase strips, ascitic fluid CRP, MPV and PDW could predict SBP in almost all cases Conclusion: MiR-155 Leukocyte esterase test, MPV, ascitic fluid CRP and culure were good diagnostic markers of SBP.

DOI

10.21608/ejmm.2022.262685

Keywords

Spontaneous Bacterial Peritonitis, Micro RNA 155, Leukocyte esterase test, MPV, CRP and culture

Authors

First Name

manal

Last Name

mosalem

MiddleName

-

Affiliation

clinical pathology, benha university of medicine, egypt

Email

manalmosalem@yahoo.com

City

-

Orcid

-

First Name

sahar

Last Name

fayed

MiddleName

mohamad

Affiliation

clinical pathlogy department,faculty of medicine ,banha univerisity

Email

saharmohamadfayed@gmail.com

City

egypt

Orcid

-

First Name

yasser

Last Name

ismail

MiddleName

mohmoud

Affiliation

clinical pathology department, faculty of medicine, banha university

Email

yasserzagazig@gmail.com

City

egypt

Orcid

-

First Name

hala

Last Name

elfeky

MiddleName

mohammed

Affiliation

hepatology, gastroenterology and infectious diseases department, faculty of medicine, banha university

Email

halaelfeky-nad@hotmail.com

City

egypt

Orcid

-

Volume

31

Article Issue

4

Related Issue

36977

Issue Date

2022-10-01

Receive Date

2022-10-01

Publish Date

2022-10-01

Page Start

45

Page End

52

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 4_Pages 45-52.pdf

PDF . 458.4kB

Link

https://ejmm.journals.ekb.eg/article_262685.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=262685

Order

262,685

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023