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262682

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Methicillin Resistant Staphylococcus aureus (MRSA) is a serious pathogen in hospitals leading to failure of treatment. MRSA colonization in pediatric patients increases the risk of severe invasive infections, while in the hospital and once they leave. Objectives: We aimed to study the molecular characters of MRSA and its prevalence among pediatric patients and healthcare workers (HCWs) in National Liver Institute (NLI), also the risk factors and mupirocin efficiency in MRSA decolonization were evaluated. Methodology: Nasal and hand swab specimens were obtained from patients and HCWs in pediatric department of NLI. MRSA detection was achieved by phenotypic methods such as cefoxitin diffusion test, VITEK2 compact system and chromogenic media, then confirmed by PCR. Susceptibility of S. aureus to different antibiotics was done by VITEK2 compact system. All isolated MRSA were subjected to SCCmec typing by multiplex PCR. Results: MRSA prevalence among HCWs was 18.9%, and among ward patients, PICU and NICU was 24.2 %, 16.7 % and 36.4 % respectively. Skin infections and less hygiene training were significant risk factors for colonization with MRSA among HCWs. Regarding antibiogram, high resistance rate was detected among MRSA isolates. Sensitivity and specificity of cefoxitin disc diffusion and VITEK2 were 97.8% and 93.3%, while that of CHROM agar was 91.3% and 80%. According to SCCmec typing, the most predominant SCCmec genotypes in the studied subjects was type III followed by type I then type IV, and the least prevalent was type V in both patients and HCWs. So, hospital acquired MRSA (36 /46) was more than community acquired MRSA strains (10/46 samples). When apply intranasal mupirocin ointment 2% regimen, a high successful decolonization rate was achieved. Conclusion: The most predominant type of SCCmec gene was type III followed by type I, which is linked to hospital acquired strains. Implementation of urgent antibiotic policy and strict infection control programs, in addition to mupirocin decolonization measures will decrease the transmission and spread of these multi drug resistant pathogens.

DOI

10.21608/ejmm.2022.262682

Keywords

MRSA, SCCmec types, HCWs, Pediatric Patients

Authors

First Name

Samah

Last Name

Awad

MiddleName

M.

Affiliation

Departement of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University Menoufia, Egypt

Email

samahawad2016@yahoo.com

City

Menoufia

Orcid

-

First Name

Azza

Last Name

Abd ElAziz

MiddleName

-

Affiliation

Departement of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University Menoufia, Egypt

Email

azzazoze@gmail.com

City

Menoufia

Orcid

-

First Name

Nermin

Last Name

adwey

MiddleName

m.

Affiliation

Departement of pediatric Hepatology, National Liver Institute, Menoufia University Menoufia, Egypt

Email

nerminadwey1121@gmail.com

City

menofia

Orcid

-

First Name

Eman

Last Name

Abou Hind

MiddleName

Sobhy

Affiliation

Microbiology department , NLI, menofia university

Email

sobhyeman0@gmail.com

City

-

Orcid

-

Volume

31

Article Issue

4

Related Issue

36977

Issue Date

2022-10-01

Receive Date

2022-10-01

Publish Date

2022-10-01

Page Start

33

Page End

43

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 4_Pages 33-43.pdf

PDF . 547.5kB

Link

https://ejmm.journals.ekb.eg/article_262682.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=262682

Order

262,682

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Details

Type

Article

Created At

23 Jan 2023