ManageChatDiscover
262680

Phenotypic and Genotypic Evaluation of Carbapenamase Producing Klebsiella Pneumoniae Isolates with Their Phylogenetic Analysis at an Egyptian University Hospital

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Widespread dissemination of carbapenem-producing Klebsiella pneumoniae (KPC) is of major concern in healthcare settings. Resistance to carbapenems involves multiple mechanisms such as the production of carbapenemases, impermeability of outer membrane and efflux pump mechanism. Objective: The aim of this study was to evaluate the prevalence of carbapenemase-producing K. pneumoniae strains among various clinical specimens obtained from different wards and to detect KPC as a mechanism of resistance. Methodology: 100 samples (55urine and 45sputum) were collected from outpatients and inpatients attending urology and chest departments in Beni Suef University Hospital aiming to isolate K. pneumoniae during the period of December 2016 to January 2018. The isolates were tested for susceptibility to ertapenem using E test. Resistant isolates were subjected to phenotypic detection of carbapenemase production by Modified Hodge Test (MHT) and molecular assessment of KPC gene by PCR. Phylogentic tree analysis was used to detect their relationship by DNA sequencing reaction. Results: K.pneumoniae were isolated from 31(31%) of the samples taken. Out of them 19(61.8%) were resistant to ertapenem by E test. By phenotypic method,17/19 (89.4%) were positive for carbapenemase by MHT; and only 13 out of them (76.4%) were confirmed as KPC by PCR. Conclusion: High rate of carbapenem-resistance in K. pneumoniae by both phenotypic and molecular methods was observed. These results warrant more firm infection control measures along with a strictly implemented antibiotic stewardship program to prevent their spread.

DOI

10.21608/ejmm.2022.262680

Keywords

Klebsiella pneumoniae, Carbapenemase, ertapenem, Modified hodge test, sequence analysis

Authors

First Name

mostAFA

Last Name

ABDEL-MOTaleb

MiddleName

SALEH

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine – Beni-Suef University.

Email

dr.m.sheemy@gmail.com

City

-

Orcid

-

First Name

Mariem

Last Name

Hamed

MiddleName

Osama

Affiliation

Department of Medical Microbiology& Immunology, Faculty of Medicine – Beni-Suef University.

Email

mariemosama2009@ymail.com

City

-

Orcid

-

First Name

Mona

Last Name

Abdel Wahab

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Medicine, Cairo University

Email

prof.mona.aa@gmail.com

City

-

Orcid

-

First Name

Abeer

Last Name

Abdel Monem

MiddleName

Ahmed

Affiliation

Department of Medical Microbiology& Immunology, Faculty of Medicine – Beni-Suef University

Email

d.abeer@hotmail.com

City

-

Orcid

-

First Name

Maha

Last Name

Fathy

MiddleName

-

Affiliation

Department of chest disease, Faculty of Medicine – Beni-Suef University

Email

mahafathy78@gmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Elgendy

MiddleName

Osama

Affiliation

Department of Microbiology, Faculty of pharmacy – Beni-Suef University

Email

ahmed.elgendy@pharm.bsu.edu.eg

City

-

Orcid

-

Volume

31

Article Issue

4

Related Issue

36977

Issue Date

2022-10-01

Receive Date

2022-10-01

Publish Date

2022-10-01

Page Start

25

Page End

31

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 4_Pages 25-31.pdf

PDF . 545.9kB

Link

https://ejmm.journals.ekb.eg/article_262680.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=262680

Order

262,680

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Phenotypic and Genotypic Evaluation of Carbapenamase Producing Klebsiella Pneumoniae Isolates with Their Phylogenetic Analysis at an Egyptian University Hospital

Details

Type

Article

Created At

23 Jan 2023