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262673

Prevalence of Vancomycin Resistance among Clinical Isolates of MRSA from Different Governorates in Egypt

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Pathogenicity and virulence

Abstract

Background: The uncontrolled use of vancomycin led to an upsurge of vancomycin-resistant S. aureus (VRSA) throughout the world. Objective: The goal of this study is to screen vancomycin resistance among MRSA isolates, determine antimicrobial resistance pattern and evaluate the distribution of virulence genes among these isolates. Methodology: A total of 127 S. aureus clinical isolates were used, MRSA isolates were identified and antimicrobial sensitivity pattern for nine antimicrobial agents from different classes was assessed. In addition, vancomycin MIC was determined by standard agar dilution method and PCR identification of vancomycin resistance encoding genes vanA and vanB was performed. Moreover, the prevalence of eight different virulence genes was determined among different vancomycin resistance categories. Results: All isolates were identified phenotypically as MRSA. However, mecA gene was detected only in 95.28% of isolates. The highest and lowest percentage of resistance was recorded for clindamycin (82.68%) and trimethoprim (11.81%), respectively. Vancomycin resistance level was 23.62% of isolates, while vanA and vanB genes were detected only in 16.67% and 10% of VRSA isolates, respectively. The highest prevalence of virulence genes was found for icaA, followed by hld, hlb, icaD, hlg, hla, tsst and cna, respectively in the tested isolates. In addition, VRSA isolates showed higher mean virulence score (MVS) of 3.6 compared to VISA and VSSA isolates. Conclusion: This study highlights the alarming problem of the increasing incidence of VRSA infections in Egypt. Therefore, there is an urgent need to rationalize vancomycin consumption and to continuously monitor the prevalence of VRSA strains.

DOI

10.21608/ejmm.2022.262673

Keywords

MRSA, VRSA vancomycin resistance, vanA, vanB, Virulence

Authors

First Name

Wesam

Last Name

Ibrahiem

MiddleName

A. M.

Affiliation

Microbiology & Immunology Dept Faculty of Pharmacy Mansoura University

Email

wesamibrahiem@gmail.com

City

-

Orcid

-

First Name

Dina

Last Name

Rizk

MiddleName

Eid

Affiliation

Microbiology & Immunology Dept, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

dinaeid2013@yahoo.com

City

-

Orcid

-

First Name

Hany

Last Name

Kenawy

MiddleName

-

Affiliation

Microbiology and Immunology Department Faculty of Pharmacy Mansoura University

Email

hanykenawy@mans.edu.eg

City

Mansoura

Orcid

0000-0002-8548-6649

First Name

Ramadan

Last Name

Hassan

MiddleName

Hassan Ebrahim

Affiliation

Microbiology & Immunology Dept, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

rhassan33@gmail.com

City

-

Orcid

-

Volume

31

Article Issue

4

Related Issue

36977

Issue Date

2022-10-01

Receive Date

2022-10-01

Publish Date

2022-10-01

Page Start

5

Page End

14

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 4_Pages 5-14.pdf

PDF . 497.4kB

Link

https://ejmm.journals.ekb.eg/article_262673.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=262673

Order

262,673

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Prevalence of Vancomycin Resistance among Clinical Isolates of MRSA from Different Governorates in Egypt

Details

Type

Article

Created At

23 Jan 2023