Background: Human papillomavirus (HPV) is the most common viral infection of the reproductive tract and the causative agent of cervical cancer and genital warts. Objectives: to study prevalence of HPV infection and type distribution of genotypes (6, 11, 16 and 18) among Egyptian females. Methodology: 65 Egyptian women were subjected to conventional Pap cytology, HPV DNA testing by polymerase chain reaction (PCR) and genotyping by multiplex PCR for genotypes (6, 11, 16 and 18) during the period from May 2018 until October 2018. Results: The prevalence of HPV among participants was (23.1%). only 20% of HPV positive cases, were infected by single HPV genotype and 80% were co-infected by more than one genotype. Conclusion: These data expand the knowledge concerning HPV prevalence and type distribution in Egypt which may help to create a national HPV prevention program.