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251706

Screening of Infectious Causes of Diarrhea and Genetic Determination of Diarrheagenic E. coli using Multiplex PCR in under 5 Years Children in Egypt

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Epidemiology

Abstract

Background: Infectious diarrhea represents a life-threatening problem among children in developing countries. Objectives: This work aimed to study bacterial, viral and parasitic causes of acute diarrhea; with genetic determination of diarrheagenic E. coli (DEC) in <5 years children. Methodology: Stool specimens were collected from 206 diarrheal children. Bacterial agents were isolated and identified by standard microbiological procedures. Multiplex PCR was done for genetic determination of DEC subtypes. ELISA was used for detection of viral and parasitic agents. Results: Stool specimens with at least single positive enteropathogen accounted for 98.5% with bacterial, viral and parasitic rates of 98.5%, 42.7% and 25.2%, respectively. Isolated bacteria were DEC (98.5%); Campylobacter (14%), Shigella (3.8%) and Salmonella (1.4%). Rota and Noroviruses showed prevalence of 32.5% and 5.3%, respectively. Conclusion: Infectious diarrhea were mostly due to bacterial agents. DEC and Campylobacter were predominant. EAEC and EPEC were the most genetically determined DEC subtypes.

DOI

10.51429/EJMM29404

Keywords

Diarrheagenic E. coli, Multiplex PCR, Rotavirus, children

Authors

First Name

Yosra

Last Name

Hassan

MiddleName

M.

Affiliation

Lecturer of Clinical and Chemical Pathology, Faculty of Medicine-Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Sahar

Last Name

Khairat

MiddleName

M.

Affiliation

Professor of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Nada

Last Name

Nawar

MiddleName

N.

Affiliation

Professor of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Maha

Last Name

Gaafar

MiddleName

M.

Affiliation

Professor of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Dina

Last Name

Hassan

MiddleName

M.

Affiliation

Associate Professor of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Mina

Last Name

William

MiddleName

-

Affiliation

MD, Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Yasmin

Last Name

ElMahdy

MiddleName

-

Affiliation

Lecturer of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Noha

Last Name

Soliman

MiddleName

S.

Affiliation

Lecturer of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt

Email

nsal18@yahoo.com

City

-

Orcid

-

Volume

29

Article Issue

4

Related Issue

35876

Issue Date

2020-10-01

Receive Date

2022-07-28

Publish Date

2020-10-01

Page Start

27

Page End

34

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 29_Issue 4_Pages 27-34.pdf

PDF . 370.5kB

Link

https://ejmm.journals.ekb.eg/article_251706.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=251706

Order

251,706

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Screening of Infectious Causes of Diarrhea and Genetic Determination of Diarrheagenic E. coli using Multiplex PCR in under 5 Years Children in Egypt

Details

Type

Article

Created At

23 Jan 2023