Background: bacteria have acquired the ability to adapt to stress conditions and developed various mechanisms of resistance; there is a need to develop new bactericidal materials. Objective: The aim of this study is to test the effect of silver nanoparticles (AgNPs) on increasing the antibiotic-susceptibility of MDR biofilm-producing Pseudomonas aeruginosa. Methodology: The antibiotic susceptibility testing of the isolates were done by the Modified Kirby- Bauer method and phenotypic detection of biofilm –forming isolates was done by the Congo Red Agar method. Results: Biofilm-production was found in16.3% of isolates and 77.6% were negative. The highest resistance rate was to piperacillin (67%), followed by ticarcillin-clavulanate (63%), while the isolates were highly sensitive to colistin (73%), polymyxin B (64%), and moderately-sensitive to meropenem (58%). Ten μL of Silver nanoparticles suspensions with size ranged from 20- 40 nm and average size 30 nm were added to the used antibiotics with retesting the isolates for their antibiotic susceptibility. Conclusion: AgNPs exhibited a strong synergistic effect with antibiotics through the significant increase of the antibiotic inhibitory zones and the improvement of the susceptibility profile of the isolates.