Background: Guillain-Barré syndrome is a serious autoimmune peripheral neuropathy, in which the immune system attacks healthy nerve cells. The reason for this condition is unknown; however it is usually triggered by an associated infection, gastrointestinal or a respiratory, and to a less extent vaccination. Several infections have been encountered preceding Guillain-Barré syndrome: Campylobacter jejuni, Influenza, Cytomegalovirus, Epstein-Barr virus, Mycoplasma pneumonia and HIV. Objectives: The aim of this research is to evaluate the etiological correlation between CMV, EBV & HHV-6 infection and Guillain Barre syndrome. Methodology: Peripheral blood samples were collected from 30 patients attending the clinic of Neurology Department, as well as 20 control subjects, to identify the presence of the CMV, EBV & HHV-6 viral genome by Real-time multiplex RCR. Results and Conclusion: This study revealed that CMV UL 32 gene was significantly higher among studied cases as compared to controls and thus may be an attributable etiological agent for triggering the disease.