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249873

Identification and characterization of a soil isolate of Chromobacterium violaceum from Egypt with potential to cause disease

Article

Last updated: 28 Dec 2024

Subjects

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Tags

Clinical microbiology

Abstract

Background: Chromobacterium violaceum (C. violaceum) is a Gram-negative bacterium mainly held to be a harmless saprophyte and only known as a biosensor strain for quorum sensing studies. However, the emergence of C. violaceum as an opportunistic pathogen is evident. In the present study, we aimed to isolate C. violaceum from soil and to test the ability of the isolates to cause infection in a murine model. Methodology: C. violaceum strain YM1 was isolated from soil using rice grains enrichment method. We identified the bacterium based on its biochemical characteristics using API 20NE as well as Vitek2. The identity of the bacterium was further confirmed on the molecular basis by phylogenetic analysis of the 16S rRNA gene sequence (GenBank accession number: MK660325). We then tested the pathogenicity of strain YM1 in a murine infection model. Results: Identification of strain YM1 revealed that it was closely related to the type strain C. violaceum ATCC 12472. Infectious doses of 104-108 cfu of YM1 were able to cause disease in mice when introduced via the intraperitoneal route. Furthermore, we provide experimental data on the antibiotic resistance phenotype of the soil isolate. Strain YM1 was resistant to multiple antibiotics to which the reference strain was susceptible. Fortunately, strain YM1 was found susceptible to fluoroquinolones; ciprofloxacin, levofloxacin, and norfloxacin. Conclusion: Here, we report on the potential clinical significance of soil isolates of C. violaceum that could be pathogenic despite their free-living style. Data presented here suggest that it is necessary to recognize C. violaceum as a suspected pathogen especially in conditions involving skin injury associated with soil.

DOI

10.21608/ejmm.2020.249873

Keywords

Quorum sensing, Chromobacterium violaceum, violacein, pathogenicity

Authors

First Name

Yasser

Last Name

Ibrahim

MiddleName

M.

Affiliation

Department of Microbiology, General Division of Basic Medical Sciences, National Organization for Drug Control and Research (NODCAR), Giza, 12611, Egypt

Email

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City

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Orcid

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First Name

Ahmed

Last Name

Abouwarda

MiddleName

M.

Affiliation

Department of Microbiology, General Division of Basic Medical Sciences, National Organization for Drug Control and Research (NODCAR), Giza, 12611, Egypt Department of Biology, Faculty of Science and Arts-Khulais, Jeddah University, Saudi Arabia

Email

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City

-

Orcid

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First Name

Nouran

Last Name

Assar

MiddleName

H.

Affiliation

Department of Microbiology, General Division of Basic Medical Sciences, National Organization for Drug Control and Research (NODCAR), Giza, 12611, Egypt

Email

drnouranhamed@hotmail.com

City

-

Orcid

-

Volume

29

Article Issue

1

Related Issue

35680

Issue Date

2020-01-01

Receive Date

2022-07-19

Publish Date

2020-01-01

Page Start

153

Page End

160

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_249873.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=249873

Order

249,873

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Identification and characterization of a soil isolate of Chromobacterium violaceum from Egypt with potential to cause disease

Details

Type

Article

Created At

23 Jan 2023