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248898

Study of Enteropathogenic Escherichia coli producing Extended Spectrum β-lactamase in Children with Acute Gastroenteritis

Article

Last updated: 28 Dec 2024

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-

Tags

Clinical microbiology

Abstract

Background: Pathogenic Escherichia coli (E. coli) represents an important etiology of acute gastroenteritis in children. Among the pathogenic E.coli, enteropathogenic E.coli  (EPEC) is associated with acute gastroenteritis in children. Objectives: The present study aims to detect the presence of t typical EPEC and atypical EPEC in children with community acquired diarrhea in age ≤ 5 years old by polymerase chain reaction for eae, bfp, Stx genes Also, to determine the presence of ESBL by phenotypic method and by polymerase chain reaction for TEM, PER, CTX-M genes. Methodology:  The isolated E.coli  were subjected to antimicrobial susceptibility test by  the disc diffusion method according to clinical laboratory standard guidelines (CLSI) .  E.coli  with resistance to cefotaxime were examined  for the presence of ESBL by the double discs method. Polymerase Chain Reaction was done to detect EPEC; eae, bfp, Stx genes and ESBL; TEM, PER, CTX-M genes. Results: A total of 290 isolates of E. coli were isolated from children with community acquired diarrhea, EPEC was identified by the presence of eaeA gene in 115 isolates of E.coli. Typical EPEC with eaeA+, bfp +, stx – genotype profile was detected in 81 (70.4%) of EPEC and atypical EPEC with eaeA+, bfp –, stx –   genotype profile was detected in 34 (29.6%) of EPEC isolates. The most frequent detected ESBL genes in EPEC were CTX-M (46.9%) followed by PER (11.3%) and TEM (9.6%.). There was a significant increase in resistance to ampicillin, cefuroxime, cefotaxime, ciprofloxacin, gentamicin, Trimethoprim/sulfamethoxazole, amikacin in EPEC compared to non EPEC. Moreover, there was a significant increase in ESBL in EPEC (P=0.0001) compared to non EPEC isolates. Conclusion: The current study highlights the presence of EPEC as a common pathogen in children with acute gastroenteritis. The typical EPEC was more common than atypical EPEC genotype. The ESBL was significantly associated with EPEC with the common gene CTX-M. Knowledge of antimicrobial resistance of EPEC and other diarrheagenic E.coli is important in selecting the appropriate therapy in serious diarrheagenic Escherichia coli infections and performing local antimicrobial guidelines.

DOI

10.21608/ejmm.2022.248898

Keywords

Enteropathogenic E coli (EPEC), Extended-spectrum β-lactamase (ESBL), acute gastroenteritis

Authors

First Name

Nashwa

Last Name

Elkasaby

MiddleName

Elsayed

Affiliation

Medical microbiology and immunology Department, faculty of medicine, Mansoura University, Egypt

Email

nashwakasby2003@yahoo.com

City

-

Orcid

0000-0002-04011-6742

First Name

Mona

Last Name

Alsayed

MiddleName

-

Affiliation

Pediatric Gastroenterology and Hepatology Unit, Mansoura University Children's Hospital, Mansoura

Email

monaa_latif@mans.edu.eg

City

-

Orcid

-

First Name

Maysaa El Sayed

Last Name

Zaki

MiddleName

Elsayed

Affiliation

Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt

Email

may_s@mans.edu.eg

City

-

Orcid

-

First Name

Asmaa

Last Name

Bakr

MiddleName

Osama

Affiliation

Clinical Pathology Department, Faculty of Medicine, Assuit University, Egypt

Email

asmaa.osman@aun.edu.eg

City

-

Orcid

-

First Name

Mohamed

Last Name

Rizk

MiddleName

Anies

Affiliation

Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egyp

Email

mohameda@mans.edu.eg

City

-

Orcid

-

Volume

31

Article Issue

3

Related Issue

35368

Issue Date

2022-07-01

Receive Date

2022-07-11

Publish Date

2022-07-01

Page Start

83

Page End

90

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 3_Pages 83-90.pdf

PDF . 480.3kB

Link

https://ejmm.journals.ekb.eg/article_248898.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=248898

Order

248,898

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Study of Enteropathogenic Escherichia coli producing Extended Spectrum β-lactamase in Children with Acute Gastroenteritis

Details

Type

Article

Created At

23 Jan 2023