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247201

Detection of Mupirocin Resistance in Methicillin- Resistant Staphylococcus aureus Isolates in an Egyptian Hospital

Article

Last updated: 23 Jan 2023

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) can be eradicated by topical mupirocin application. Mupirocin resistance, on the other hand, is becoming more widespread. Objective: The present work aims to compare conventional and molecular approaches to detect the prevalence of mupirocin resistance in MRSA isolates from clinical and nasal samples, as well as to investigate their susceptibility to other antibiotics. Methodology: Our study included 60 MRSA non-duplicate isolates, 14 from surgical wounds, 16 from urinary tract infections from patients hospitalized to Suez- Canal University Hospital in Ismailia, Egypt, and 30 nasal swabs from health care workers. The minimum inhibitory concentrations (MICs) for MRSA isolates to mupirocin were determined using the E-test method, and PCR targeting the mupA gene was performed. Results: Six isolates out of 60 MRSA isolates (10%) showed high-level mupirocin resistance, while just one strain (1.6%) showed low- level mupirocin resistance. Four of the six MRSA isolates with high levels of mupirocin resistance carried the mupA gene. All seven mupirocin-resistant isolates (11.6%) were isolated from nasal swabs. MRSA strains resistant to mupirocin were more resistant to tetracycline, chloramphenicol, gentamycin, ciprofloxacin, and trimethoprim-sulfamethoxazole than mupirocin-susceptible ones. Conclusion: The high prevalence of mupirocin resistance in MRSA strains at our hospital is alarming. As a result, frequent testing of MRSA for mupirocin resistance is recommended even in settings where mupirocin is not used prophylactically.

DOI

10.21608/ejmm.2022.247201

Keywords

Mupirocin resistance, Methicillin resistance, Staphylococcus aureus

Authors

First Name

Samaa

Last Name

Taha

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Suez- Canal University, Ismailia, Egypt; Department of Microbiology, College of Medicine, Taif University, Al-Taif, Saudi Arabia

Email

samaa_taha@yahoo.com

City

-

Orcid

-

First Name

Noha

Last Name

Kamel

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Medicine, Suez- Canal University, Ismailia, Egypt

Email

-

City

-

Orcid

-

First Name

Lobna

Last Name

Metwally

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Suez- Canal University, Ismailia, Egypt; Department of Microbiology, College of Medicine, Taif University, Al-Taif, Saudi Arabia

Email

-

City

-

Orcid

-

Volume

31

Article Issue

3

Related Issue

35368

Issue Date

2022-07-01

Receive Date

2022-07-01

Publish Date

2022-07-01

Page Start

51

Page End

55

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 3_Pages 51-55.pdf

PDF . 186.5kB

Link

https://ejmm.journals.ekb.eg/article_247201.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=247201

Order

247,201

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

-

Details

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Article

Created At

23 Jan 2023