Subjects
-Abstract
Background: Hypervirulent Klebsiella pneumoniae (hvKp) cause multiple dangerous infections. HvKp can be distinguished from classical K. pneumoniae (cKp) by hyper-mucoviscosity. Presence of multiple genes contribute to hypervirulent phenotype of K. pneumoniae as mucoviscosity-associated gene A (magA) and regulator of mucoid phenotype A (rmpA). Objectives: to detect hvKp frequency among strains of k.pneumoniae by using string test phenotypically, to use multiplex polymerase chain reaction for rmpA and magA genes detection. Methodology: Fifty isolates of k.pneumoniae, were obtained from hospitalized patients in El-zohour Hospital. Conventional identification and susceptibility to antimicrobials were performed. Results: Most strains were isolated from samples of urine. Thirty strains out of fifty (60%) were hvKp by using string test. magA and rmpA were present in 14 isolates (28%) and 29 isolates (58.0%) respectively among k.pneumoniae isolates. Out of thirty hvKp strains, magA and rmpA genes were present in 14 stains (46.7%) and 25 strains (83.3%) respectively and 12 strains (40%) were coharboring both genes and were string test positive. Conclusions: high frequency of hvKp among clinical samples. Prompt detection of hvKp in different clinical settings is needed through establishing effective diagnostic tools. String test is a sensitive tool for rmpA gene detection. Establishment of both proper preventive measures and antimicrobial stewardship programs may be helpful to overcome spreading of multidrug resistant hvKp.
DOI
10.21608/ejmm.2022.247186
Keywords
Hypervirulent k. pneumonia (hvKP), string test, mucoviscosity-associated gene A (magA) and regulator of mucoid phenotype A (rmpA)
Authors
Affiliation
Medical Microbiology & Immunology Faculty of Medicine Ain Shams University
Email
monaadelkhattab@yahoo.com
MiddleName
-Affiliation
Medical Microbiology &Immunology Faculty of Medicine, Misr University for Science and Technology
Email
rody_hager@yahoo.com
City
-Orcid
-Link
https://ejmm.journals.ekb.eg/article_247186.html
Detail API
https://ejmm.journals.ekb.eg/service?article_code=247186
Type
New and original researches in the field of Microbiology.
Publication Title
Egyptian Journal of Medical Microbiology
Publication Link
https://ejmm.journals.ekb.eg/
MainTitle
Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals