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247173

Microbiological Profile, Biofilm Formation and Antimicrobial Resistance Pattern of Pathogenic Bacteria Isolated from Chronic Ulcerative Lesions

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Chronically infected wounds represent a major public health problem which is associated with increased morbidity and mortality, particularly with the global threat of antimicrobial resistance. Objectives: Isolation of pathogenic micro-organisms from chronic ulcerative lesions, identification and evaluation of their biofilm formation and antimicrobial resistance pattern. Methodology: This study was carried out on 50 specimens, collected from chronic ulcerative lesions of patients admitted to the Inpatient and Outpatient Surgical Units, Tanta university hospitals. Pathogenic organisms were isolated and identified. Antimicrobial susceptibility was determined, and biofilm formation assay was performed using a tissue culture plate method. Results: The most frequently isolated organisms were Pseudomonas spp. (26.42%), Klebsiella spp. (24.52%) then E. coli (16.98%). All E. coli isolates showed resistance to aztreonam and Ampicillin /sulbactam. Also, all Klebsiella isolates showed resistance to ciprofloxacin, levofloxacin and Ceftazidime. While 92.86% of Pseudomonas isolates showed resistance to Ceftazidime. About 58.5 % of isolated pathogens were biofilm producers with Pseudomonas spp. were the most frequently biofilm producers. Conclusion: The isolated pathogens showed high rate of resistance to most of the tested antimicrobial agents, with high rate of biofilm formation among most of isolates.

DOI

10.21608/ejmm.2022.247173

Keywords

Chronic ulcerative lesions, Antimicrobial resistance, Biofilm

Authors

First Name

Alyaa

Last Name

Alsaadi

MiddleName

Gamal

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt

Email

dr.alyaa93@yahoo.com

City

Tanta

Orcid

-

First Name

Eman

Last Name

hegazy

MiddleName

elsayed

Affiliation

microbiology faculty of medicine Tanta univerisity

Email

eman.hegazi@med.tanta.edu.eg

City

Tanta

Orcid

0000-0001-9263-1894

First Name

Tarek

Last Name

Shoukr

MiddleName

Gamal

Affiliation

Plastic and Reconstructive surgery, faculty of medicine ,tanta university, Egypt

Email

tarek.shokr@med.tanta.edu.eg

City

Tanta

Orcid

-

First Name

Raghda

Last Name

Talaat

MiddleName

Zaki

Affiliation

Medical Microbiology and immunology, Faculty of medicine, Tanta university, Egypt

Email

raghda.talat@med.tanta.edu.eg

City

Tanta

Orcid

-

Volume

31

Article Issue

3

Related Issue

35368

Issue Date

2022-07-01

Receive Date

2022-07-01

Publish Date

2022-07-01

Page Start

9

Page End

16

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 3_Pages 9-16.pdf

PDF . 515.4kB

Link

https://ejmm.journals.ekb.eg/article_247173.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=247173

Order

247,173

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Microbiological Profile, Biofilm Formation and Antimicrobial Resistance Pattern of Pathogenic Bacteria Isolated from Chronic Ulcerative Lesions

Details

Type

Article

Created At

23 Jan 2023