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247169

Co-occurrence of blaNDM-1 and blaOXA-48 among carbapenem resistant Enterobacteriaceae isolates causing bloodstream infections in Alexandria, Egypt

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Bloodstream infections are serious ailments, that require prompt appropriate treatment. This is challenging with the growing number of multi-drug resistant organisms. Objective: The aim of this study was to genotypically characterize the carbapenem resistance among cephalosporin resistant Enterobacteriaceae isolates causing bloodstream infections. Methodology: Twenty-eight enterobacterial isolates resistant to ceftazidime and cefepime causing bloodstream infections were collected. Their identification was confirmed using Vitek-2 system (Biomerieux, France), and their susceptibility pattern was assessed using disk diffusion method. They were genotypically tested for the presence of blaSHV, blaCTX-M as well as blaTEM. Furthermore, carbapenem resistant isolates were genotypically examined to detect blaKPC, blaIMP, blaNDM-1, blaVIM and blaOXA-48 genes. Results: The enterobacterial isolates were identified as Klebsiella pneumoniae and Escherichia coli. Of these, (85.71%) were carbapenem resistant. Genotypically blaCTX-M, blaSHV and blaTEM were detected in (85.71%), (89.29%) and (64.29%) of the twenty-eight isolates, respectively. Moreover, (66.67%) and (62.50%) of the carbapenem resistant isolates possessed blaOXA-48 and blaNDM-1, respectively. Only one harbored blaVIM. Moreover, blaIMP and blaKPC were not detected among these isolates. Ten (35.71%) of the isolates harbored beta-lactamases genes belonging to three beta-lactamases classes (A, B and D). Conclusion: The co-occurrence of different beta-lactamases belonging to diverse classes and the co-existence of blaOXA-48 and blaNDM-1 among isolates causing bloodstream infections is quite alarming and necessitates prompt action.

DOI

10.21608/ejmm.2022.247169

Keywords

blaCTX-M, blaNDM-1, blaOXA-48, carbapenemases, ESBL

Authors

First Name

Amira

Last Name

ElBaradei

MiddleName

-

Affiliation

Department of Microbiology and Immunologgy, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt.

Email

amiraelbaradei@gmail.com

City

Alexandria

Orcid

0000-0001-6813-7896

Volume

31

Article Issue

3

Related Issue

35368

Issue Date

2022-07-01

Receive Date

2022-07-01

Publish Date

2022-07-01

Page Start

1

Page End

7

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 3_Pages 1-7.pdf

PDF . 252.0kB

Link

https://ejmm.journals.ekb.eg/article_247169.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=247169

Order

247,169

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Co-occurrence of blaNDM-1 and blaOXA-48 among carbapenem resistant Enterobacteriaceae isolates causing bloodstream infections in Alexandria, Egypt

Details

Type

Article

Created At

23 Jan 2023