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Association of Polymorphisms of X-ray Repair Cross-complementing 1 (XRCC1) Protein and Aflatoxin B1 (AFB1) in Egyptian Patients with Hepatocellular Carcinoma

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Hepatocellular carcinoma (HCC) is the most prevalent malignancy affecting developing populations Aflatoxin B1 (AFB1), an established carcinogen produced by certain strains of fungi, particularly Aspergillus parasiticus and Aspergillus flavus.  The carcinogenic effects of AFB1 in hepatocellular carcinoma (HCC) are ascribed to AFB1-DNA adduct formation with p53 gene mutation. Objective: The present study aimed was to investigate the prevalence of single nucleotide polymorphism (SNP) in rs 25487 of X-ray cross complementing group 1 protein (XRCC1) in association with AFB1 in patients with hepatocellular carcinoma (HCC). Methodology: The study included 100 patients with HCC and 100 healthy control subjects. Blood samples were withdrawn from each subject and SNP in rs 25487 XRCC1 was estimated by real time polymerase chain reaction (PCR) and determination of blood levels of AFB1. Results: There was a dramatic increase in the level of aflatoxin B1 in patients (1.02±0.7ng/gm) compared to control subjects (0.8±0.6ng/gm), P=0.01. A significant increase in mutant genotypes CT and TT was found in HCC patients (43% and 7%, respectively) compared to control subjects (27% and 1%, respectively), with a notable increase in the dominant genotype CC in healthy controls (72%) compared to patients with HCC (50%), P=0.002. There was also a statistically significant increase in C allele (85.5%)  in the control subjects compared to patients with HCC (71.5%) with a significant increase in T allele in patients (28.5%) compared to control subjects (14.5%), P=0.001. Furthermore, a significant difference in level of aflatoxin B1 was detected in patients with different genotypes of rs25487 XRCC1, with high levels of aflatoxin B1 found in patients with CT genotype (1.2±0.5), P=0.002. Conclusion: This study revealed the relationship of SNP of XRCC1 rs25487 with hepatocellular carcinoma, showing a significant association between this SNP and the increased level of aflatoxin B1 in patients with HCC.

DOI

10.21608/ejmm.2022.229024

Keywords

HCC, XRCC1 rs25487, Aflatoxin B1

Authors

First Name

Radwa

Last Name

Rabea

MiddleName

Ahmed

Affiliation

clinical pathology department,faculty of medicine, Beni-Suef university

Email

radwarabea23@yahoo.com

City

Beni Suef

Orcid

0000-0002-2073-6555

First Name

Nashwa

Last Name

Elkouly

MiddleName

-

Affiliation

Internal Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt Taibah University, King Saudi Arabia

Email

-

City

Cairo

Orcid

-

First Name

Dina

Last Name

Elhammady

MiddleName

-

Affiliation

Tropical Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

Mansoura

Orcid

-

First Name

Doaa

Last Name

Gad

MiddleName

F.

Affiliation

Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

Mansoura

Orcid

-

First Name

Maysaa

Last Name

Zaki

MiddleName

E.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

Mansoura

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-03-26

Publish Date

2022-04-04

Page Start

115

Page End

119

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 115-119.pdf

PDF . 180.4kB

Link

https://ejmm.journals.ekb.eg/article_229024.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=229024

Order

18

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Association of Polymorphisms of X-ray Repair Cross-complementing 1 (XRCC1) Protein and Aflatoxin B1 (AFB1) in Egyptian Patients with Hepatocellular Carcinoma

Details

Type

Article

Created At

23 Jan 2023