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228835

Granulysin (GNLY) Gene Polymorphism and serum Interleukin 23 level in Psoriasis patients in Benha University Hospital

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Psoriasis is a chronic, non infectious, relapsing inflammatory skin disease caused by genetic, immunological and other factors in the environment, affecting 2–5% of the world's population . Granulysin is an anti-microbial peptide that contributes to local amplification of inflammation. The activation and  maintenance of the T helper 17 pathway is the essential role of of IL23 in the pathogenesis of  psoriasis. Objectives: Evaluation of the relation of granulysin (GNLY) gene single nucleotide polymorphism to psoriasis pathogenesis, measurement of interleukin23 (IL23) level in sera of cases of psoriasis and its relation to disease severity. Methodology: our study was done on 50 subjects divided into 2 groups:30 psoriasis patients, selected from Outpatients Clinics of Dermatology, Venerology and Andrology Department of Benha University Hospitals, and 20 apparent healthy control subjects of matched sex and age. Four ml venous blood samples were collected from psoriasis patients and control subjects to genotype GNLY rs7908 (C/G) and GNLY rs10180391(C/T) polymorphisms  by Polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP)-based analysis and detection of interleukin (IL23) level using ELISA. Results: GNLY rs7908 CC genotype was significantly lower in psoriasis patients (6.7%) than in the control group (30%). The difference between patients and controls for GNLY rs10180391 allele frequen­cies was not statistically significant. IL23 level increased significantly with increased severity of psoriasis. Conclusion: GNLY rs 7908 gene polymorphismhad a significant relation with severity of psoriasis. IL23 level was directly proportional to the severity of psoriasis.

DOI

10.21608/ejmm.2022.228835

Keywords

Granulysin, Gene polymorphism, IL23 level, Psoriasis

Authors

First Name

Wafaa

Last Name

El Mosallamy

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

-

City

Benha

Orcid

-

First Name

Amany

Last Name

Mustafa

MiddleName

I.

Affiliation

Department of Dermatology, Venerology and Andrology, Faculty of Medicine, Benha University, Egypt

Email

-

City

Benha

Orcid

-

First Name

Roshdan

Last Name

Arafa

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

-

City

Benha

Orcid

-

First Name

Sally

Last Name

Saleh

MiddleName

Aly

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Kafr Elsheikh University,Egypt

Email

sallysaleh17@yahoo.com

City

Kafr Elsheikh

Orcid

-

First Name

Mysa

Last Name

Mostafa

MiddleName

S.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

maysa.alsayed@fmed.bu.edu.eg

City

Benha

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-03-24

Publish Date

2022-04-03

Page Start

109

Page End

114

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 109-114.pdf

PDF . 455.2kB

Link

https://ejmm.journals.ekb.eg/article_228835.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=228835

Order

17

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Granulysin (GNLY) Gene Polymorphism and serum Interleukin 23 level in Psoriasis patients in Benha University Hospital

Details

Type

Article

Created At

23 Jan 2023