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228830

Evaluation of OXA-48 in Carbapenem Resistant Gram-Negative Isolates

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Carbapenems production represents the most important mechanism that affects carbapenem activity. Among the Enterobacteriaceae, three molecular types of carbapenems are of major clinical importance since they are frequently associated with severe nosocomial infections. These are KPC (Ambler class A), NDM (class B) and OXA-48 (class D). Objective: The aim of the work is to determine the prevalence of carbapenem resistance and carbapenems encoding genes among clinical K. pneumoniae isolates obtained from patients at Intensive Care Units (ICUs) of Mansoura Oncology Institute. Methodology: This study included twenty-four Carbapenem resistant gram-negative bacilli (CRG), samples were collected from the Oncology Center in Mansoura University, Egypt, between October 2014 and February 2017. Samples were collected from different body sites such as: swabs, blood, urine, pus aspirate. Results: OXA-48 gene was positive in 16 samples and negative in 8 samples. Among the OXA-48 positive samples, 8 samples were positive by Modified Hodge test- Ertapenem disk, 5 samples were positive by Modified Hodge test- Meropenem disk, 3 samples were positive by  combined disk test-Meropenem boronic acid Class A and 5 samples were positive by combined disk test- Imipenem EDTA Class B. Among the OXA-48 negative samples, 2 samples were positive by Modified Hodge test- Ertapenem disk, 3 samples were positive Modified Hodge test- Meropenem disk and 3 samples were positive by combined disk test- Imipenem EDTA Class B. Conclusion: Antibiotic resistance is considered a global health problem which is caused by misuse of antibiotics with the lack of the new antibiotic's development.

DOI

10.21608/ejmm.2022.228830

Keywords

OXA-48, carbapenem, resistant, Gram-Negative

Authors

First Name

Morad

Last Name

Agha

MiddleName

Salah

Affiliation

Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Email

moradagha2@gmail.com

City

-

Orcid

-

First Name

Rateb

Last Name

Abbas

MiddleName

-

Affiliation

Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Email

ratebyoussef@gmail.com

City

-

Orcid

-

First Name

Mahmoud

Last Name

Ismail

MiddleName

-

Affiliation

Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Email

abdelmongyd@yahoo.com

City

-

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-03-18

Publish Date

2022-04-03

Page Start

95

Page End

101

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 95-101.pdf

PDF . 314.4kB

Link

https://ejmm.journals.ekb.eg/article_228830.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=228830

Order

15

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Evaluation of OXA-48 in Carbapenem Resistant Gram-Negative Isolates

Details

Type

Article

Created At

23 Jan 2023