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228827

A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background & objectives: Stenotrophomonas maltophilia (S. maltophilia) emerged as an important opportunistic nosocomial multidrug resistant pathogen. This study aimed to investigate S. maltophilia strains to determine their virulence factors, antimicrobial resistance pattern, detect integrons and reveal their genotypic relatedness. Methodology: S. maltophilia clinical isolates were subjected to antimicrobial susceptibility testing using the VITEK-2 compact system (BioMérieux). Biofilm production, hemolytic, protease and lipase activity were tested.  Polymerase Chain Reaction (PCR) for integrase 1 and 2 genes and Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR were done. Results:S. maltophilia constituted 0.8% of gram-negative non-fermenters. All strains were biofilm producers. All strains were susceptible to trimethoprim/sulfamethoxazole. Class 1 integron was detected in five (35.7%) strains. ERIC PCR showed high genetic diversity between the strains. Conclusions: Although multiple virulence factors were detected, strains were still susceptible to the recommended antimicrobials. ERIC PCR was found to be valuable in detecting genetic diversity among S. maltophilia strains being easy, rapid, cheap and available technique. 

DOI

10.21608/ejmm.2022.228827

Keywords

Stenotrophomonas maltophilia, MALDITOF, Virulence factors, ERIC PCR, Epidemiology

Authors

First Name

Marwa

Last Name

Meheissen

MiddleName

Ahmed

Affiliation

Medical Microbiology & Immunology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

Email

marwa.meheissen@alexmed.edu.eg

City

-

Orcid

0000-0002-7122-4760

First Name

Mona

Last Name

Morsi

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, University of Alexandria, Egypt

Email

morsirg@gmail.com

City

Alexandria

Orcid

-

First Name

Mabrouka

Last Name

Hamad

MiddleName

-

Affiliation

Medical Microbiology and Immunology, Microbiology Department, Faculty of Medicine, Sirte University, Sirte, Libya.

Email

mabrukaahmad232@yahoo.com

City

-

Orcid

-

First Name

May

Last Name

Raouf

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, University of Alexandria, Egypt.

Email

dr_maymoheb@yahoo.com

City

-

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-02-14

Publish Date

2022-04-03

Page Start

69

Page End

76

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 69-76.pdf

PDF . 254.0kB

Link

https://ejmm.journals.ekb.eg/article_228827.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=228827

Order

12

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

A one year single-center experience on Stenotrophomonas maltophilia strains in Alexandria, Egypt

Details

Type

Article

Created At

23 Jan 2023