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228825

Colistin Susceptibility Testing Methods for Carbapenem Resistant Acinetobacter baumannii, A Comparative Study

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Acinetobacter baumanii is an emerging nosocomial pathogen that is associated with several infections that are extremely difficult to treat. Extensive and pan-drug resistance to multiple antibiotic classes is rising leading to limited treatment options and making colistin a last resort. Practical and accurate susceptibility testing methods are a mandatory demand for rapid and reliable sensitivity reporting of colistin. Objective: The aim was to test colistin sensitivity of carbapenem-resistant Acinetobacter baumanii, using different commonly used testing methods in the routine microbiology laboratory as disc diffusion, broth microdilution, E-test and Vitek-2 system. Results: This research showed that disc diffusion and E-test failed to meet the CLSI criteria with high very major errors (100% and 33%). Comparative evaluation between the three studied antibiotic susceptibility methods to colistin against broth microdilution as a reference method, showed 100% categorical agreement with Vitek-2. Conclusion: We concluded that besides the reference broth microdilution method, Vitek-2 is an automated alternative reliable option to test the bacterial susceptibility to colistin in the laboratory.

DOI

10.21608/ejmm.2022.228825

Keywords

Acinetobacter baumanii, Colistin, Vitek, E-test

Authors

First Name

Sara

Last Name

Asser

MiddleName

Lotfy

Affiliation

Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University

Email

sara.asar@alexmed.edu.eg

City

Alexandria

Orcid

0000-0002-9937-9473

First Name

Dina

Last Name

Kholeif

MiddleName

Aly

Affiliation

Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University

Email

dina424@hotmail.com

City

Alexandria

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-03-03

Publish Date

2022-04-03

Page Start

63

Page End

67

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 63-67.pdf

PDF . 366.9kB

Link

https://ejmm.journals.ekb.eg/article_228825.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=228825

Order

11

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Colistin Susceptibility Testing Methods for Carbapenem Resistant Acinetobacter baumannii, A Comparative Study

Details

Type

Article

Created At

23 Jan 2023