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Emergence of Fluoroquinolone resistance and carbapenemase plasmids in Enterobacter cloacae isolated from Egyptian Pediatric Hospital

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Enterobacter cloacae cause opportunistic infections that are frequently associated with multidrug resistance (MDR). Co-carriage of plasmid mediated quinolone resistance (PMQR) genes and carbapenemases worsened the problem of resistance. Objectives: The current work aimed to detect the frequency of PMQR in E. cloacae as well as the co-carriage of carbapenemase resistance determinants in Egypt. Methodology: Fourteen E. cloacae isolates were collected from children suffered from gastroenteritis admitted at Pediatric Hospital at Assiut University. Identification and antimicrobial susceptibility were done by Vitek-2® system. Detection of PMQR and carbapenemases was done by PCR. Conjugation experiment was performed to test the transmissibility of the resistance determinants. Results: PMQR genes were detected in 3/14 (21.4%) of E. cloacae isolates. qnrB and qnrS were detected in 2/14 (14.3%) and 3/14 (21.4%) of the isolates, respectively. Two E. cloacae isolates co-harbored qnrB and qnrS. Neither qnrA, qnrD nor aac (6՛)-Ib was detected. Carbapenemase genes were detected in 7/14 (50%) E. cloacae isolates; 𝑏𝑙𝑎NDM-1, 𝑏𝑙𝑎OXA-48 and 𝑏𝑙𝑎VIM-2 were detected in 6/14(42.9%), 2/14 (14.3%) and 1/14(7.1%) of E. cloacae isolates respectively. Only one isolate (7.1%) co-harbored 𝑏𝑙𝑎NDM-1, 𝑏𝑙𝑎VIM-2 and 𝑏𝑙𝑎OXA-48 genes. Conclusion: PMQR and carbapenemases determinants are common among E. cloacae isolates in Egypt with the co-existence of multiple resistance determinants in the same isolate. All transmitted determinants suggest their presence on transmissible plasmids.

DOI

10.21608/ejmm.2022.228621

Keywords

Enterobacter cloacae, PMQR, Carbapenemase, Co-existence, conjugation

Authors

First Name

Aliaa

Last Name

Ghandour

MiddleName

Mahmoud

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.

Email

aliaaghandour@yahoo.com

City

Assiut

Orcid

-

First Name

Safy

Last Name

Hadiya

MiddleName

-

Affiliation

Assiut International Center of Nanomedicine, Al-Rajhy Liver Hospital, Assiut University, Egypt

Email

ph.s.h.1989@gmail.com

City

Assiut

Orcid

-

First Name

Rania

Last Name

Nasseif

MiddleName

Gamal

Affiliation

Women's Health Hospital, Assiut University, Assiut, Egypt

Email

raniagamalclinical@gmail.com

City

Assiut

Orcid

-

First Name

Marwa

Last Name

Sabet

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx University, New-Assiut, Egypt.

Email

marwasabet@gmail.com

City

Assiut

Orcid

-

First Name

Rania

Last Name

Bakyr

MiddleName

-

Affiliation

Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.

Email

rbakry.md@gmail.com

City

Assiut

Orcid

-

First Name

shaban

Last Name

Sror

MiddleName

-

Affiliation

Gastroenterology and Hepatology Unit, Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt.

Email

gem123shabansror@gmail.com

City

-

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-02-19

Publish Date

2022-04-01

Page Start

37

Page End

43

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 37-43.pdf

PDF . 428.6kB

Link

https://ejmm.journals.ekb.eg/article_228621.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=228621

Order

7

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Emergence of Fluoroquinolone resistance and carbapenemase plasmids in Enterobacter cloacae isolated from Egyptian Pediatric Hospital

Details

Type

Article

Created At

23 Jan 2023