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228619

Co-existence of blaOXA-48, rmtB and armA among Klebsiella pneumoniae Isolates Causing Respiratory Tract Infections in Alexandria, Egypt

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Klebsiella pneumoniae is frequently implicated in numerous health-care infections, including respiratory tract infections. Aminoglycosides are among the available options to manage such infections. Hence, resistance to aminoglycosides in K. pneumoniae isolates is a clear source of concern. Objective: The aim of our study was to investigate the presence of 16S rRNA methyltransferases genes among aminoglycoside resistant K. pneumoniae isolates causing respiratory tract infections. Methodology: K. pneumoniae isolates resistant to gentamycin, tobramycin and amikacin were collected from samples obtained from respiratory tract infections from different hospitals in Alexandria, Egypt. Antimicrobial susceptibility testing was performed using disc diffusion method. Genotypically, we investigated the presence of different 16S rRNA methyltransferases encoding genes (armA, rmtA, rmtB, rmtC and rmtD) as well as other genes (aac(6')Ib and blaOXA-48). Results: Thirty K. pneumoniae isolates resistant to aminoglycosides were collected, and they were also resistant to carbapenems. Fourteen out of the 30 (46.67%) isolates harbored armA gene and two of these 30 isolates (6.67%) carried rmtB. However, rmtA, rmtC and rmtD were not detected. Fifteen (50%) of the isolates harbored aac(6')Ib gene. On the other hand, blaOXA-48 gene was present in 29 (96.67%) out of our 30 isolates. Conclusions: All isolates that were resistant to aminoglycosides were found to be multi-drug resistant (MDR) isolates. Eighteen isolates harbored at least one gene conferring resistance to aminoglycosides. Fourteen of these harbored genes encoding 16S rRNA methyltransferases. The co-occurrence of different resistance genes among many of our isolates represents a clear threat. 

DOI

10.21608/ejmm.2022.228619

Keywords

ARMA, aac(6')Ib, blaOXA-48, Klebsiella pneumoniae, rmtB

Authors

First Name

Amira

Last Name

ElBaradei

MiddleName

-

Affiliation

Department of Microbiology and Immunologgy, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt. Alexandria University Hospital, Alexandria University, Alexandria, Egypt

Email

amiraelbaradei@gmail.com

City

Alexandria

Orcid

0000-0001-6813-7896

First Name

Sherine

Last Name

Shawky

MiddleName

-

Affiliation

Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt

Email

sherineshawky@hotmail.com

City

Alexandria

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-02-01

Publish Date

2022-04-01

Page Start

23

Page End

28

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 23-28.pdf

PDF . 307.6kB

Link

https://ejmm.journals.ekb.eg/article_228619.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=228619

Order

5

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Co-existence of blaOXA-48, rmtB and armA among Klebsiella pneumoniae Isolates Causing Respiratory Tract Infections in Alexandria, Egypt

Details

Type

Article

Created At

23 Jan 2023