Subjects
-Abstract
Background: Infertility is caused by numerous anatomical and hormonal factors; however, the role of ascending colonization from vaginal microbiota in infertility is not properly addressed. C.albicans, the most common yeast retrieved from vagina is associated with 61.53% cases of infertility while C.species is associated with 38.46% 1. Intrauterine Candida colonization and its effect on ICSI are still under study and need to be explored further. Objective: This work aimsis to detect the effect of Candida colonization of the uterus in patients undergoing ICSI cycle and clinical pregnancy rate. Methodology: Menstrual blood flow sample at day two induction cycles is subjected to culture and identification of growing organisms. Growth and identification was correlated to Success of ICSI. Predetermined criteria were set up for selection of patients. Results: 69.2% of females with positive growth had failed the ICSI trial with p value 0.04, while 94.4% of patients with no culture growth had successful ICSI with p value 0.0001. Samples with growth of mixed Candida species with bacterial isolates either gram positive and /or gram negative showed 100% failed trials. Samples showed pure growth of Candida albicans or Candida spp. had positive ICSI with p value <0.001. Conclusion: Pure Candida colonization does not seem to affect ICSI outcome; in contrary, mixed Candida with gram positive and/or negative colonization affect pregnancy rate negatively. This could be due to microbial interactions and increasing the ability of Candida to turn into the pathogenic state when mixed with other microorganisms. Screening of females and administration of antifungals and/or antibiotics before ICSI is recommended.
DOI
10.21608/ejmm.2022.228614
Keywords
microbiota, upper genital tract, ICSI, Clinical pregnancy rate
Authors
Affiliation
Department of Clinical and chemical pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
Email
amirafarouk77@gmail.com
Orcid
-MiddleName
-Affiliation
Department of Gynecology and Obstetrics, Faculty of medicine, Cairo University, Cairo, Egypt
Orcid
-Affiliation
prof of microbiology department ,faculty of science benha uni
Orcid
-MiddleName
-Affiliation
Department of Botany and Microbiology, Faculty of science, Banha University, Qalyuia, Egypt
Orcid
-MiddleName
-Affiliation
Department of Gynecology and Obstetrics, Faculty of medicine, Cairo University, Cairo, Egypt
Email
radwafahmy@yahoo.com
Orcid
-Link
https://ejmm.journals.ekb.eg/article_228614.html
Detail API
https://ejmm.journals.ekb.eg/service?article_code=228614
Type
New and original researches in the field of Microbiology.
Publication Title
Egyptian Journal of Medical Microbiology
Publication Link
https://ejmm.journals.ekb.eg/
MainTitle
The Effect of Intrauterine Candida Colonization on Pregnancy Outcome Following Intra-cytoplasmic Sperm Injection (ICSI)