Beta
ManageSearchChatDiscover
228613

Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Multi-Drug Resistant (MDR) P. aeruginosa is a major health problem in burn infection. Exotoxin A gene plays an important role in pathogenesis of burn infections Objective: the isolation of MDR p.aeruginosa from infected burns and Detection of Exotoxin-A (Exo-A) gene in MDR P. aeruginosa isolates using conventional Polymerase Chain Reaction (PCR). Methods: The study was carried out on 200 samples of burn patients in a form of burn swabs, these patients admitted to Burn Units at Tanta university Hospitals. PCR was done on isolated MDR P.aeruginosa for detection of exotoxin- A gene. Results: P. aeruginosa was the most common organism isolated from burn infection with a percentage of 32.5% .96.3% of them contained the EXO-A gene while only two cases (3.3%) didn't contain the gene. Conclusion: EXO-A gene is an important virulence factor of P.aeruginosa infected burns. MDR P. aeruginosa incidence increased in hospitals.

DOI

10.21608/ejmm.2022.228613

Keywords

Burn wound, P.aeruginosa, MDR, EXO-A gene

Authors

First Name

laila

Last Name

sultan

MiddleName

taher

Affiliation

medical microbiology and immunology , faculty of medicine, Tanta university

Email

lailasultan39@gmail.com

City

Tanta

Orcid

-

First Name

kareman

Last Name

Eshra

MiddleName

A

Affiliation

Department of Surgery and burn Faculty of Medicine, Tanta University, Egypt

Email

hashem-ayad@tanta.med.edu.eg

City

Tanta

Orcid

-

First Name

Fawkia

Last Name

Mahgoub

MiddleName

Mohamed

Affiliation

medical microbiology and immunology , faculty of medicine, Tanta university

Email

fawkia.mahgoub@tanta.med.edu.eg

City

Tanta

Orcid

-

First Name

kareman

Last Name

Eshra

MiddleName

Ahmed

Affiliation

medical microbiology and immunology , faculty of medicine, Tanta university

Email

drkaremaneshra2004@hotmail.com

City

Tanta

Orcid

-

Volume

31

Article Issue

2

Related Issue

32749

Issue Date

2022-04-01

Receive Date

2022-01-06

Publish Date

2022-04-01

Page Start

7

Page End

11

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 2_Pages 7-11.pdf

PDF . 368.9kB

Link

https://ejmm.journals.ekb.eg/article_228613.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=228613

Order

2

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital

Details

Type

Article

Created At

23 Jan 2023