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212002

Bacterial Profile in Urine, Burns, and Wounds in Diabetic and non-Diabetic Patients

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: The risks that threaten diabetic patients as a result of bacterial infection exceed than those in normal persons. This is clearly seen in skin, urinary tract and wound infections, which require special medical care and increase the cost of treatment, especially when these infections are accompanied by wide resistance to many antibiotics and the possession of resistance genes that can be transmitted to other bacteria. Objectives: Are todetermine the prevalence of antibiotics resistant bacteria in diabetes. Methodology: One hundred and thirty bacteria were isolated from urine, burns and wounds from diabetes and non-diabetes in two hospitals in Alexandria, Egypt. The were screened for their antibiotics resistance. These isolates were tentatively identified related to Staphylococcus aureus and named M22 “diabetic skin", Pseudomonas aeruginosa named M76 “diabetic skin" and E. coli which named M113 “diabetic urine". These three isolates were selected for further investigations, since they achieved the highest resistance to traditional antibiotics. Isolate M22 was resistant to Methicillin, isolate M76 and M113 showed more resistance to Ampicillin. PCR was carried out for detection of Mec A gene in S. aureus, and β-lactamase gene in Pseudomonas aeruginosa and E. coli. Results: The presence of Methicillin resistant gene (MRSA) have confirmed by PCR as well as the ampicillin resistant genes in Pseudomonas aeruginosa and E. coli. The resistant genes were confirmed by the presence of distinguished bands at 858 bp and 850 bp respectively. Conclusion: Special cares should be considered and taken to prevent or reduce bacterial infections of diabetics.  

DOI

10.21608/ejmm.2022.212002

Keywords

diabetes, Non diabetes, antibiotics, genome, MRSA, Amp. β-lactam

Authors

First Name

Magdy

Last Name

Abu-Gharbia

MiddleName

A.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Sohag University, Sohag, 82524, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Abd El-Fattah

MiddleName

M.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Sohag University, Sohag, 82524, Egypt

Email

-

City

-

Orcid

-

First Name

Khalid

Last Name

AbdelRehim

MiddleName

Abdalla Ali

Affiliation

Botany dept., Faculty of science, Sohag university - Sohag - Egypt

Email

khalidfp7@gmail.com

City

Sohag

Orcid

-

Volume

31

Article Issue

1

Related Issue

29807

Issue Date

2022-01-01

Receive Date

2021-12-13

Publish Date

2022-01-01

Page Start

113

Page End

119

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 1_Pages 113-119.pdf

PDF . 356.5kB

Link

https://ejmm.journals.ekb.eg/article_212002.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=212002

Order

16

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Bacterial Profile in Urine, Burns, and Wounds in Diabetic and non-Diabetic Patients

Details

Type

Article

Created At

23 Jan 2023