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211962

Effect of Silver Nanoparticles on Biofilm Producing Multi Drug Resistant Uro-pathogenic E. coli isolated from Catheterized Patients in Mansoura University Hospital

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Clinical microbiology

Abstract

Background:Uro-pathogenic E. coli (UPEC) is considered one of the members of the extra-intestinal pathogens, that predominantly cause urinary tract infections. Objectives: Assessment of the prevalence of MDR UPEC in catheterized patients admitted into Mansoura University Hospital, then detection of biofilm production by isolated MDR UPEC strains, and finally testing the effect of silver nanoparticles on biofilm producing ability of the isolated strains. Methodology: 155 urine samples were processed to detect E. coli strains, Antibiotic susceptibility profile of resulting E. coli isolates was determined by Kirby- Bauer disc diffusion method to select MDR UPEC strains, then detection of biofilm production using tissue culture plate method among isolated MDR UPEC strains, and finally evaluation of anti-biofilm activity of silver nanoparticles (AgNPs) by re-detection of biofilm formation after incubation with various concentrations of silver nanoparticles. Results: Out of these urine samples, 35 isolates (22.6%) revealed E. coli. Out of them, 34 isolates (97.1%) were MDR E. coli. 29 isolates (85%) of MDR UPEC were biofilm producers. The antibiofilm effect was observed in dose dependent manner. Silver nanoparticles with concentration of 100μg/ml, recorded maximum antibiofilm effect (63.22%) followed by concentrations of 75, 50, and 25μg/ml which were able to eliminate the biofilm formation of MDR UPEC on the plate surface by (59.91%), (55.24%), (43.61%) respectively.Conclusion: Silver nanoparticles play a major role in the inhibition of biofilm formation by MDR UPEC.
Abbreviations: (AgNPs, Silver nanoparticles; CAUTI, Catheter associated urinary tract infection; MDR, Multidrug resistance; TCP, Tissue culture plate; TSB, Trypticase soy broth; UPEC, Uro-pathogenic E. coli).

DOI

10.21608/ejmm.2022.211962

Keywords

Biofilm, CAUTI, MDR UPEC, silver nanoparticles

Authors

First Name

Eman

Last Name

Elbehairy

MiddleName

Nashat

Affiliation

Microbiology and immunology department faculty of medicine mansoura university

Email

emannashat91@gmail.com

City

Mansoura

Orcid

-

First Name

Magda

Last Name

El Nagdy

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

First Name

Ghada

Last Name

Weefky

MiddleName

F.

Affiliation

Department of Anesthesia and Surgical ICU, Faculty of medicine, Mansoura university, Mansoura, Egypt

Email

-

City

-

Orcid

-

First Name

Yasmin

Last Name

Nabiel

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

Volume

31

Article Issue

1

Related Issue

29807

Issue Date

2022-01-01

Receive Date

2021-11-28

Publish Date

2022-01-01

Page Start

63

Page End

68

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 1_Pages 63-68.pdf

PDF . 403.9kB

Link

https://ejmm.journals.ekb.eg/article_211962.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=211962

Order

9

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Effect of Silver Nanoparticles on Biofilm Producing Multi Drug Resistant Uro-pathogenic E. coli isolated from Catheterized Patients in Mansoura University Hospital

Details

Type

Article

Created At

23 Jan 2023