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211949

Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Spontaneous bacterial peritonitis is the development of an infection of the ascitic fluid in the peritoneum, with no identifiable source of the infection in patients with liver failure. ascitic fluid neutrophil count is more than 250⁄mm3. calprotectin in Ascitic fluid may help in detection of neutrophil count < 250 cells/mm3, it has an effective role in detection of SBP and this will be a rapid treatment and good prognosis. Objectives: Evaluation of  the calprotectin in ascitic fluid  as  rapid  marker for diagnosis of SBP. Methodology: our study was done on 40 patients in Hepatology and Gastroenterology Department of Benha University divided into 2 groups 20 patients were SBP group and other 20 patients were non SBP group. 5ml of ascitic fluid was collected from  patient in sterile blood culture bottles under complete aseptic condition then cultured on automated blood culture system (Bact/ALERT 3D) .Serum Calprotectin was measured in ascitic  fluid by using commercially available  quantitative sandwich enzyme-linked  immune sorbent assays. Results: SBP was in significant association with higher TLC in AF and higher frequency of positive culture results when compared to non SBP group (p < 0.001 for each).Among all studied cases, median calprotectin level was 0.602 ng/dL. SBP was significantly associated with higher level of calprotectin when compared to non SBP group .Calprotectin showed positive significant correlation with TLC in ascitic fluid. Conclusion: calprotectin in ascitic fluid is an excellent rapid  marker for diagnosis of spontaneous bacterial peritonitis in patients with chronic Liver disease.

DOI

10.21608/ejmm.2022.211949

Keywords

Spontaneous Bacterial Peritonitis, Calprotectin, ELISA

Authors

First Name

Ahmed

Last Name

Shafik

MiddleName

O.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Elgazzar

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

ahmed.algazar01@fmed.bu.edu.eg

City

-

Orcid

-

First Name

Ahmed

Last Name

Elgazar

MiddleName

S.

Affiliation

Department of Hepatology and Gastroentrology, Faculty of Medicine, Benha University, Egypt.

Email

ahmed.elgazar@fmed.bu.edu.eg

City

-

Orcid

-

First Name

Mysa

Last Name

Mostafa

MiddleName

S.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

maysa.alsayed@fmed.bu.edu.eg

City

-

Orcid

-

First Name

Marwa

Last Name

Elaraby

MiddleName

-

Affiliation

Department of Medical Microbiology and immunology, Faculty of Medicine, Benha University, Egypt.

Email

drmarwaelaraby@gmail.com

City

Benha

Orcid

-

Volume

31

Article Issue

1

Related Issue

29807

Issue Date

2022-01-01

Receive Date

2021-11-05

Publish Date

2022-01-01

Page Start

47

Page End

54

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 1_Pages 47-54.pdf

PDF . 411.5kB

Link

https://ejmm.journals.ekb.eg/article_211949.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=211949

Order

7

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease

Details

Type

Article

Created At

23 Jan 2023