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211939

Diagnosis of Helicobacter pylori infection in Assiut University Hospital and medical laboratories by using non-invasive tests

Article

Last updated: 05 Jan 2025

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-

Tags

Clinical microbiology

Abstract

Background: Helicobacter pylori is a gram negative bacterium and considered as one of the causative factors of  gastritis and peptic ulceration.The exact route of transmission is still unknown. H. pylori infection was highly prevalent worldwide. Objectives:  to detect H. pylori infection rate by different tests including ELIZA tests for anti H. pylori antibodies (IgM, IgG, IgA) and H. pylori antigen, to isolate H. pylori by bacterial culture, to evaluate antimicrobial susceptibility of bacteria and to estimate bacterial infection rate according to clinical symptoms of patients. Methodology: This study was done on 130 stool samples and 130 blood samples collected from 130 patients attending Gastroenterology Department of Assiut University Hospital and some special medical laboratories, collected during the period from March 2019 to February 2020. Stool samples were processed to detect H. pylori antigen titers and to isolate H. pylori bacteria, blood samples were processed to detect anti H. pylori antibodies. Antimicrobial susceptibility of H. pylori was evaluated to ten antibiotics by disc diffusion method. Results: H. pylori infection rate was 86.1% by bacterial culture, 81.5% by anti-H. pylori IgG antibodies test , 48.5% by anti-H. pylori IgA antibodies test, 13.8% by anti-H. pylori IgM antibodies test, 76.9% by H. pylori antigen test. 39.2% of  H. pylori infected patients had abdominal pain. Bacterial strains were resistant to several antibiotics. Conclusion: we concluded that anti-H. pylori IgG antibodies and H. pylori antigen tests were the preferable tests for diagnosis of H. pylori infection. According to statistical analysis, these results were more reliable and valuable. Abdominal pain was the most common symptom in H. Pylori infection. ciperofloxacin and amoxicillin were the most effective antibiotics for inhibition of bacteria. 

DOI

10.21608/ejmm.2022.211939

Keywords

Helicobacter pylori, Anti H. pylori antibodies, H. pylori antigen, antibiotics resistance

Authors

First Name

Magdy

Last Name

Abu-Gharbia

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Sohag University

Email

-

City

-

Orcid

-

First Name

Michael

Last Name

Agban

MiddleName

N.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University

Email

-

City

-

Orcid

-

First Name

Ayman

Last Name

Hamouda

MiddleName

M.A.

Affiliation

Medicinal and Aromatic Plants Department, Director of Horticulture Research Institute, Agriculture Research Center, Giza

Email

-

City

-

Orcid

-

First Name

Rasha

Last Name

Abdelmasieh

MiddleName

Z

Affiliation

Researcher in Medicinal and Aromatic Plants Department, Horticulture Research Institute, Agriculture Research Center, Giza

Email

rzaher094@gmail.com

City

Sohag

Orcid

-

Volume

31

Article Issue

1

Related Issue

29807

Issue Date

2022-01-01

Receive Date

2021-11-05

Publish Date

2022-01-01

Page Start

39

Page End

45

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 31_Issue 1_Pages 39-45.pdf

PDF . 232.9kB

Link

https://ejmm.journals.ekb.eg/article_211939.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=211939

Order

6

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Diagnosis of Helicobacter pylori infection in Assiut University Hospital and medical laboratories by using non-invasive tests

Details

Type

Article

Created At

23 Jan 2023