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202189

Detection of Biofilm and Siderophore Encoding Genes Implicated in the Pathogenesis of Klebsiella pneumoniae Isolated from Different Clinical Specimens

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Biofilm and siderophores protect K. pneumoniae, making them resistant to antimicrobials and increase the ability to evade immune system. Objective: The aim of the present study was to detect some of the virulence genes responsible for biofilm formation and siderophore production implicated in the pathogenesis of K. pneumoniae. Methodology: Crystal violet method was used to detect biofilm phenotypically, hypermucoviscosity by string test and virulence genes were detected by PCR. Results: Phenotypically, biofilm formation was positive in 76% and negative in 24%.  Genotypically, FimH and ycfm were detected in 100% of isolates while mrkD in 98%. Kfu, entB and iutA were found in 100%, 68% and 34% respectively. Phenotypically by string test 66% of isolates were classified as hypermucoviscous, while 34% as classical. rmpA gene was detected in 52% of isolates. Conclusion: FimH, mrkD, ycfm, Kfu and iutA genes are implicated in multisystemic infection of K. pneumoniae. Enterobactin gene is more predominant than other siderophore genes.

Keywords

Klebsiella pneumoniae, Siderophores, Biofilm, Antimicrobial resistance

Authors

First Name

Iman

Last Name

Naga

MiddleName

Salah

Affiliation

Microbiology Department, Medical Research Institute, Alexandria University, Egypt

Email

iman.naga@alexu.edu.eg

City

Alexandria

Orcid

-

Volume

30

Article Issue

1

Related Issue

28170

Issue Date

2021-01-01

Receive Date

2020-11-07

Publish Date

2021-01-01

Page Start

101

Page End

108

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 1_Pages 101-108.pdf

PDF . 483.8kB

Link

https://ejmm.journals.ekb.eg/article_202189.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=202189

Order

13

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection of Biofilm and Siderophore Encoding Genes Implicated in the Pathogenesis of Klebsiella pneumoniae Isolated from Different Clinical Specimens

Details

Type

Article

Created At

23 Jan 2023