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202182

Detection and identification of Mycobacterium species

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

typing and identification

Abstract

Background: Successful diagnosis and effective treatment for mycobacterial infections are mainly depending on a rapid and sensitive identification method. Objective: To detect and identify the Mycobacterium species. Methodology:  PCR and LCD-microarry techniques were compared with the classical methods of Ziehl-Neelsen staining (ZN) and culturing. Two primers based on two conservative regions within the mycobacterium 16S rRNA gene were designed and amplified a DNA fragment of about 1350 bp for both complex of Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM). Results: Regarding to the standard method of culture, 57 positive individuals were identified out of 100 urine samples. The PCR showed 96.30 % sensitivity and 96.70% specificity, while ZN gave Se = 67.50 % and Sp = 100 %. The LCD-microarray analysis exhibited 100 % sensitivity and specificity. One species of MTB was determined as M. tuberculosis and positively represented by 12.3% (n=7). Five species of NTM were determined and represented as M. kansasii 36.8 % (n=21), M. celatum 21 % (n=12), M. gordonae 12.2% (n=7), M. chelonae 10.5 % (n=6), and M. phlei 7% (n=4). Conclusion: The results recommended utilizing the simple and rapid PCR method for early mycobacteria detection. Also, the fast LCD-microarray protocol is very beneficial for identification and differentiation between MTB and of NTM species.

Keywords

mycobacterium, Ziehl-Neelsen staining, PCR, Microarray

Authors

First Name

Asaad

Last Name

Gaber

MiddleName

Abdel-Salam

Affiliation

Medical analysis fellow, Urology & Nephrology Center, Mansoura University, Mansoura, Egypt.

Email

asaadgaber@yahoo.com

City

Mansoura

Orcid

-

Volume

30

Article Issue

1

Related Issue

28170

Issue Date

2021-01-01

Receive Date

2020-11-02

Publish Date

2021-01-01

Page Start

79

Page End

86

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 1_Pages 79-86.pdf

PDF . 427.3kB

Link

https://ejmm.journals.ekb.eg/article_202182.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=202182

Order

10

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection and identification of Mycobacterium species

Details

Type

Article

Created At

23 Jan 2023