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202179

Evaluating the Performance of Four SARS-CoV-2 Commercial Chemiluminescence Immunoassays

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background: COVID-19 is a pandemic of serious global threat that forced test developers to flood the market with various diagnostic assays that have been independently validated. Objectives: This study aims at assessing the performance of four SARS-CoV-2 chemiluminescence immunoassays. Methodology: The present study included sera from 96 Polymerase Chain Reaction (PCR)–confirmed COVID-19 cases collected at 2 time periods (5-9 days and 9-14 days post symptom onset) during patient follow-up, and 30 control sera from COVID-19 PCR–negative individuals. All sera were tested for SARS-CoV-2 antibodies using four high-throughput commercially available chemiluminescence immunoassays: YHLO Biotech Co, Ltd China (IgM and IgG); Abbott, Abbott USA (IgG); Roche, Roche US (total: IgM, IgG, and IgA), and Ortho, Ortho Clinical Diagnostics, USA (total and individual IgG). Results: For the detection of total antibodies (IgM, IgG, and IgA), the highest sensitivities were for Ortho followed by Roche assays (91.6% and 84.3% in 5-9 days period, respectively) raised to 96.8% and 92.7% in 9-14 days respectively with significant difference P-value Conclusion: Ortho assay showed the best performance in detecting total antibodies with perfect match to Roche assay, while Abott assay was the best performing in detecting individual IgG with perfect match to Ortho assay rendering them to be efficient diagnostic tools.

Keywords

COVID-19, SARS-CoV-2 antibodies, Chemiluminescence, Immunoassays

Authors

First Name

Noha

Last Name

Soliman

MiddleName

Salah

Affiliation

Lecturer of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt.

Email

nsal18@yahoo.com

City

Cairo

Orcid

-

Volume

30

Article Issue

1

Related Issue

28170

Issue Date

2021-01-01

Receive Date

2020-10-21

Publish Date

2021-01-01

Page Start

71

Page End

77

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 1_Pages 71-77.pdf

PDF . 411.5kB

Link

https://ejmm.journals.ekb.eg/article_202179.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=202179

Order

9

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Evaluating the Performance of Four SARS-CoV-2 Commercial Chemiluminescence Immunoassays

Details

Type

Article

Created At

23 Jan 2023