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202173

The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background: Cases with pure neural leprosy (PNL) are difficult in their diagnosis and usually loss the early management opportunity. Objective: to evaluate Real time PCR and high resolution ultrasonography of peripheral nerves as diagnostic tools in PNL. Methodology The study included 25 suspected PNL and 25 healthy controls. All patients were subjected to ultrasonography cross-sectional area (CSA) measurement. Fine needle aspiration (FNA) was obtained, and followed by Ziehl-Neelsen (ZN)staining, and real time PCR. Results: ZN staining demonstrated M leprae bacilli in only 8 cases while their DNA was detected in 92 % cases (Sensitivity of both were 100%, and 44.4%, respectively). Only the left median and right and left posterior tibial nerves CSA measurements showed good performance in distinguishing patients of PNL.  Median nerve cut off=15.5 mm2, with a sensitivity of 72%), and right and left posterior tibial nerves CSA Cut Off were 12, 11.5 mm2 with sensitivity of 76-84%. Conclusions: ultrasonography of suspected nerves and real time PCR of nerve aspirates are simple accurate tests for diagnosis of PNL.

Keywords

Pure neural leprosy, Real Time, PCR, High resolution ultrasound

Authors

First Name

Nissreen

Last Name

Elbadawy

MiddleName

E.

Affiliation

Assistant Professor of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University

Email

nissreenbadawy77@gmail.com

City

Zagazig

Orcid

-

Volume

30

Article Issue

1

Related Issue

28170

Issue Date

2021-01-01

Receive Date

2020-10-14

Publish Date

2021-01-01

Page Start

45

Page End

52

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 1_Pages 45-52.pdf

PDF . 510.7kB

Link

https://ejmm.journals.ekb.eg/article_202173.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=202173

Order

6

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy

Details

Type

Article

Created At

23 Jan 2023