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199832

Seroepidemiology, Antimicrobial Susceptibility and Virulence Characteristics of Clinical Klebsiella pneumoniae Isolates in Mansoura University Hospitals

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Last updated: 05 Jan 2025

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Tags

Epidemiology

Abstract

Background: Klebsiella pneumoniae is one of the crucial causes of nosocomial and community-acquired infections that can result in various infections in human. Objectives: The present study aim to investigate the prevalence of capsular serotypes, antimicrobial susceptibility and virulence characteristics of K. pneumoniae isolated from different Mansoura University Hospitals. Methodology: K. pneumoniae isolates were collected from different clinical sources at Mansoura University Hospitals. The antimicrobial susceptibility to 14 different antibiotics was determined by disk diffusion method. The capsular serotypes were assessed by quelling test. Serum resistance, haemagglutination, biofilm, lipase, protease and lecithinase enzymes production were assessed phenotypically. Moreover, four virulence genes (rmpA, fimH, kfuBC and wabG) were detected by PCR. The genetic relatedness among isolates was investigated using ERIC-PCR molecular typing. Results: Seventy-three isolates were confirmed as K. pneumoniae. The vast majority of isolates demonstrated MDR patterns (72.6%) including a high resistance rate to the beta-lactam antibiotics (ampicillin: 98.6%, amoxicillin-clavulanic acid: 97.26 %, piperacillin: 97.26 %, amoxicillin: 93.15% and cefotaxime: 94.52%). K1 and K2 were the main serotypes found among the isolates, K1 serotype was the predominant (79.45%).   It was found that serum resistance was the highest detected virulence factor among isolates (95.9%) and lipase was the lowest detected factor (19.2%). Haemagglutination was detected in 63% of the isolates especially from rectal swab (83.3%) and sputum (72.7%). The biofilm formation was detected mainly among urine and blood isolates.  rmpA, fimH, kfuBC and wabG genes were harbored by 20.5%, 92%, 66% and 94.5% of isolates, respectively. ERIC- PCR showed high genetic diversity (100%, typability, Simpson's index of diversity= 1).  Conclusion: The current study revealed the high antibiotic resistance levels, pathogenic potential, and genetic diversity among K. pneumoniae isolated from different clinical sourceswhich is considered a serious health problem that necessitates interventions to control its spread.

Keywords

K. pneumoniae, Serotypes, Virulence, Molecular Typing

Authors

First Name

Aya

Last Name

Elasmer

MiddleName

H.

Affiliation

Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

Email

-

City

Mansoura

Orcid

-

First Name

Mohammed

Last Name

Ibrahim

MiddleName

Y.

Affiliation

Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt School of Biological Sciences, University of Cambridge

Email

-

City

Mansoura

Orcid

-

First Name

Dina

Last Name

Rizk

MiddleName

E.

Affiliation

Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

Email

dena@mans.edu.eg

City

Mansoura

Orcid

0000-0003-1609-1878

Volume

30

Article Issue

1

Related Issue

28170

Issue Date

2021-01-01

Receive Date

2020-09-21

Publish Date

2021-01-18

Page Start

9

Page End

18

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 1_Pages 9-18.pdf

PDF . 1.2MB

Link

https://ejmm.journals.ekb.eg/article_199832.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=199832

Order

2

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Seroepidemiology, Antimicrobial Susceptibility and Virulence Characteristics of Clinical Klebsiella pneumoniae Isolates in Mansoura University Hospitals

Details

Type

Article

Created At

23 Jan 2023