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198932

Effect of subinhibitory concentrations of selected antibiotics and propolis on pyocyanin and biofilm production among Pseudomonas aeruginosa isolates in Alexandria, Egypt

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Pseudomonas aeruginosa is a highly virulent microorganism that is implicated in various types of infections. It is armed with an arena of virulence factors that is mostly controlled by quorum sensing. The pigment pyocyanin and biofilm formation are of the important defense mechanisms used by the organism to establish infection. Objective: detection and quantification of biofilm mass and pyocyanin along with effect of subinhibitory concentrations of antibiotics on their magnitude. Methodology: In the present study, fifty Pseudomonas aeruginosa isolates were obtained from clinical laboratories from all over Alexandria governorate, Egypt. The isolates were tested for certain quorum sensing dependent virulence factors and the effect of subinhibitory concentrations of certain antibiotics, in addition  propolis extract, was assayed. Results: the sub MIC of selected antibiotics and propolis inhibited pyocyanin production. On the other hand, they had variable effects on the formed biofilm mass. Conclusion: the effect of sub inhibitory concentrations either on biofilm formation or pyocyanin production is very important to be tested and followed to predict bacterial behavior and assist in tailoring therapeutic regimen.

DOI

10.21608/ejmm.2021.198932

Keywords

Pyocyanin, Biofilm forming capacity, phzS, phzM, SubMIC antibiotics

Authors

First Name

Nevine

Last Name

Seiffein

MiddleName

L.

Affiliation

Department of Microbiology and immunology, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Ghada

Last Name

Ali

MiddleName

Hani

Affiliation

Department of Microbiology and immunology, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt

Email

ghada_han@yahoo.com

City

Alexandria

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-09-29

Publish Date

2021-10-10

Page Start

129

Page End

137

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 129-137.pdf

PDF . 493.2kB

Link

https://ejmm.journals.ekb.eg/article_198932.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=198932

Order

16

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Effect of subinhibitory concentrations of selected antibiotics and propolis on pyocyanin and biofilm production among Pseudomonas aeruginosa isolates in Alexandria, Egypt

Details

Type

Article

Created At

23 Jan 2023