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197469

High-Level Aminoglycoside and Vancomycin Resistance in Enterococcus spp. Isolated from Hospital Acquired Infections, Ismailia, Egypt

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

chemotherapy

Abstract

Background: Multidrug resistant Enterococci (MDR) are important hospital acquired infections (HAIs). Objectives: This study aimed to determine the genetic basis of resistance to aminoglycosides and vancomycin in enterococci strains isolated from hospitalized patients with HAIs. Methodology: The disc diffusion method was used to detect high-level aminoglycoside resistance (HLAR) by using gentamicin (120 𝜇g) and streptomycin (300 𝜇g) discs. Nested PCR was performed for the six-aminoglycoside resistance genes. Vancomycin resistance genes were discovered by conventional PCR. Results: In a total of 100 Enterococcus isolates, aac(6')-Ie-aph(2")-Ia gene was found in 30 strains, 25 of them were E.faecalis and the remaining were E.faecium. aph(3'). The presence of high levels of gentamycin and streptomycin resistance was more common in strains carrying aac(6')-1e-aph(2'')-1a gene. We detected vanA gene in 9 isolates and van C in 15 isolates. vanB was not detected.  E.faecium strains containing the vanA gene were shown to be resistant to vancomycin by MIC (MIC > 32 μg/mL), nitrofurantoin, ampicillin and penicillin (P<0.001). Van C was detected in 12 isolates of E.faecalis, 1 isolate of E.facium and 2 isolates of E.durans. E.faecalis carrying vanC gene was more resistant to aminoglycoside than vanC negative E.faecalis.. Conclusion: E. faecalis has been seen to be more widespread than other Enterococcus species in our research. we found that the aac(6')-Ie-aph(2")-Ia and aph(3')-IIIa genes are more frequently found than other genes.

DOI

10.21608/ejmm.2021.197469

Keywords

HLAR, nested PCR, van A, E. faecalis

Authors

First Name

Rania

Last Name

Kishk

MiddleName

M.

Affiliation

Associate prof. of Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

rankishk@yahoo.com

City

Ismailia

Orcid

-

First Name

Nader

Last Name

Nemr

MiddleName

-

Affiliation

Endemic and Infectious diseases department, Faculty of Medicine, Suez Canal University

Email

nadernemr@med.suez.edu.eg

City

-

Orcid

-

First Name

Nourhan

Last Name

Soliman

MiddleName

H.

Affiliation

Clinical Pathology department, Faculty of Medicine, Suez Canal University

Email

norhan_hassan@med.suez.edu.eg

City

-

Orcid

-

First Name

Eman

Last Name

Riad

MiddleName

-

Affiliation

Internal medicine department, Chest Unit, Faculty of Medicine, Suez Canal University

Email

emanriad@med.suez.edu.eg

City

-

Orcid

-

First Name

Mona

Last Name

Ahmed

MiddleName

M.

Affiliation

Internal Medicine Department, Faculty of Medicine, Suez Canal University

Email

mona_mostafa@med.suez.edu.eg

City

-

Orcid

-

First Name

Nashaat

Last Name

Soliman

MiddleName

M.

Affiliation

Endemic and Infectious diseases department, Faculty of Medicine, Suez Canal University

Email

-

City

-

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-09-17

Publish Date

2021-10-02

Page Start

113

Page End

119

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 113-119.pdf

PDF . 399.2kB

Link

https://ejmm.journals.ekb.eg/article_197469.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197469

Order

14

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

High-Level Aminoglycoside and Vancomycin Resistance in Enterococcus spp. Isolated from Hospital Acquired Infections, Ismailia, Egypt

Details

Type

Article

Created At

23 Jan 2023