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197468

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

typing and identification

Abstract

Background: Matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) technology is a technique which allows microbial identification in clinical microbiology laboratories based on microbial protein profiles. A promising application of the MALDI-TOF technique comprises carrying out identification from direct urine samples. Objectives: The aim of this study is to evaluate the performance of MALDI-TOF MS system for the rapid identification of clinically relevant urinary tract pathogens in urine samples to the species level. Methodology: two hundred (200) urine samples with findings suggestive of urinary tract infections (UTI) were identified directly by MALDI-TOF MS, and same samples were identified from culture on MALDI-TOF MS. 73 colonies from 73 samples were further identified by Bd Phoneix and PCR. Results: MALDI-TOF MS directly from samples identified 174 samples (91 to the species level 83 to genus level), while MALDI-TOF MS after culture identified 191 samples (187 to the level of species, and 4 to genus level). In addition, 9 samples gave no results from the direct method and no growth in culture. The sensitivity and specificity of direct method compared to after culture method was 91.1% and 100%, respectively. Colonies of 73 samples were detected by the BD Phoneix automated identification system and by PCR. The results were identical among the three systems. Conclusion: The ability of MALDI-TOF MS's to detect microorganisms from urine samples directly can provide early diagnosis of UTIs and avoid the inadequate or unnecessary empirical antimicrobial treatment. 

DOI

10.21608/ejmm.2021.197468

Keywords

MALDI-TOF MS, Urinary tract infection, BD Phoenix

Authors

First Name

Ahmed

Last Name

Mahmoud

MiddleName

G. T.

Affiliation

Specialist of Medical Microbiology, Central lab of armed force, Egypt

Email

dr.a.gad81@gmail.com

City

-

Orcid

-

First Name

Lamia

Last Name

Fathy

MiddleName

F.

Affiliation

Professor of Medical Microbiology and Immunology Department, Ain Shams University, Egypt

Email

lamiaazzam@gmail.com

City

-

Orcid

-

First Name

Nehal

Last Name

Fahim

MiddleName

M.A.

Affiliation

Professor of Medical Microbiology and Immunology Department, Ain Shams University, Egypt

Email

nehal.fahim@gmail.com

City

-

Orcid

-

First Name

Walaa

Last Name

El-Sadek

MiddleName

Abd El-Latif

Affiliation

Medical Microbiology &Immunology ,Ain Shams University ,Cairo ,Egypt

Email

loulla_latif@yahoo.com

City

cairo

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-09-01

Publish Date

2021-10-02

Page Start

103

Page End

112

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 103-112.pdf

PDF . 769.0kB

Link

https://ejmm.journals.ekb.eg/article_197468.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197468

Order

13

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections

Details

Type

Article

Created At

23 Jan 2023